Panicucci Gabriele, Barreto Pedro, Herzog Max, Lichtenauer Sophie, Schwarzländer Markus, Pedersen Ole, Weits Daan A
Experimental and Computational Plant Development, Institute of Environment Biology, Utrecht University, Padualaan 8, Utrecht 3584 CH, Netherlands.
Plant Energy Biology, Institute of Plant Biology and Biotechnology, University of Münster, Schlossplatz 8, Münster 48143, Germany.
Plant Physiol. 2024 Dec 23;197(1). doi: 10.1093/plphys/kiae624.
Our understanding of how low oxygen (O2) conditions arise in plant tissues and how they shape specific responses has seen major advancement in recent years. Important drivers have been (1) the discovery of the molecular machinery that underpins plant O2 sensing; and (2) a growing set of dedicated tools to define experimental conditions and assess plant responses with increasing accuracy and resolution. While some of those tools, such as the Clark-type O2 electrode, were established decades ago, recent customization has set entirely new standards and enabled novel research avenues in plant hypoxia research. Other tools, such as optical hypoxia reporters and O2 biosensor systems, have been introduced more recently. Yet, their adoption into plant hypoxia research has started to generate novel insight into hypoxia physiology at the tissue and cellular levels. The aim of this update is to provide an overview of the currently available and emerging tools for O2 hypoxia measurements in plants, with an emphasis on high-resolution analyses in living plant tissues and cells. Furthermore, it offers directions for future development and deployment of tools to aid progress with the most pressing questions in plant hypoxia research.
近年来，我们对植物组织中低氧（O₂）条件如何产生以及它们如何塑造特定反应的理解取得了重大进展。重要的推动因素包括：（1）发现了支撑植物O₂感知的分子机制；（2）越来越多的专用工具，这些工具能够以更高的准确性和分辨率定义实验条件并评估植物反应。虽然其中一些工具，如克拉克型O₂电极，是几十年前建立的，但最近的定制设定了全新的标准，并为植物缺氧研究开辟了新的研究途径。其他工具，如光学缺氧报告基因和O₂生物传感器系统，则是最近才引入的。然而，它们在植物缺氧研究中的应用已开始在组织和细胞水平上对缺氧生理学产生新的见解。本综述的目的是概述目前可用于植物O₂缺氧测量的工具以及正在出现的工具，重点是对活植物组织和细胞进行高分辨率分析。此外，它还为未来工具的开发和应用提供了方向，以帮助解决植物缺氧研究中最紧迫的问题。
