Perl A, Láng I, Gonzalez-Cabello R, Filep J, Nékám K, Gergely P, Fehér J
Immunopharmacology. 1986 Feb;11(1):39-45. doi: 10.1016/0162-3109(86)90063-9.
The effects of cyclosporin A, prostaglandin E1 and indomethacin were studied on lectin-dependent cell-mediated cytotoxicity (LDCC) against adherent HEp-2 human epipharynx carcinoma target cells. LDCC activity by human peripheral blood lymphocytes was evaluated by detachment from the monolayer of [3H]thymidine-prelabelled HEp-2 cells in a 24-h assay at 50:1 effector:target cell ratio in the presence of 25 micrograms/ml concanavalin A. Under these conditions, but without concanavalin A, considerable natural cell-mediated cytotoxicity was not elicited although LDCC was significantly augmented in the presence of concanavalin A. Addition of both cyclosporin A (0.1, 1.0 or 10 micrograms/ml) and prostaglandin E1 (10(-8), 10(-7) or 10(-6) M) dose-dependently suppressed LDCC activity. Indomethacin (0.1, 1.0 or 10 micrograms/ml) did not in itself influence LDCC although suppression of LDCC by cyclosporin A, but not prostaglandin E1, was abrogated in the presence of indomethacin. Similar to indomethacin, acetyl salicylic acid also reversed the inhibition of LDCC by cyclosporin A. In parallel experiments, cyclosporin A elicited a more than two-fold increase of prostaglandin E production under LDCC assay conditions as measured by radioimmunoassay. Contrary to LDCC, depression of concanavalin A induced blastogenesis by cyclosporin A was not influenced by indomethacin, suggesting that the inhibition by cyclosporin A of LDCC and concanavalin A-induced blastogenesis proceed via different mechanisms.
研究了环孢素A、前列腺素E1和吲哚美辛对针对贴壁HEp-2人上咽癌细胞靶细胞的凝集素依赖性细胞介导的细胞毒性（LDCC）的影响。通过在24小时试验中以50:1的效应细胞:靶细胞比例在存在25微克/毫升伴刀豆球蛋白A的情况下从[3H]胸腺嘧啶预先标记的HEp-2细胞单层上脱离，来评估人外周血淋巴细胞的LDCC活性。在这些条件下，但无伴刀豆球蛋白A时，虽然在伴刀豆球蛋白A存在下LDCC显著增强，但未引发可观的自然细胞介导的细胞毒性。添加环孢素A（0.1、1.0或10微克/毫升）和前列腺素E1（10(-8)、10(-7)或10(-6) M）均剂量依赖性地抑制LDCC活性。吲哚美辛（0.1、1.0或10微克/毫升）本身不影响LDCC，尽管在吲哚美辛存在下环孢素A对LDCC的抑制作用被消除，但前列腺素E1对LDCC的抑制作用未被消除。与吲哚美辛类似，乙酰水杨酸也逆转了环孢素A对LDCC的抑制作用。在平行实验中，通过放射免疫测定法测得，在LDCC测定条件下，环孢素A使前列腺素E的产生增加了两倍多。与LDCC相反，吲哚美辛不影响环孢素A对伴刀豆球蛋白A诱导的母细胞生成的抑制作用，这表明环孢素A对LDCC和伴刀豆球蛋白A诱导的母细胞生成的抑制作用是通过不同机制进行的。
