Abraham E C, Elseweidy M M
Hemoglobin. 1986;10(2):173-83. doi: 10.3109/03630268609046443.
In vivo glycosylated components of Hb S were isolated from red cell hemolysates of sickle cell anemia patients by application of affinity chromatographic and cation exchange chromatographic techniques. The total glycosylated fraction (GHb) of the whole hemolysate, Hb SIc, Hb So-glycosylated (formed mostly by glycosylation epsilon-NH2 groups of lysyl residues), and Hb So-nonglycosylated fractions were isolated in this manner. GHb contained about 33% Hb SIc and 42% Hb So (Hb So-glycosylated) and the rest was glycosylated Hb F and Hb A2. As expected, the binding of 2,3-DPG was affected only in Hb SIc and not in Hb So-glycosylated. Hb SIc and Hb So-glycosylated had higher solubility in concentrated phosphate solutions and had higher minimum gelling concentrations than the non-glycosylated form of Hb So. These effects are interpreted to be due to modification by glycosylation of specific sites that are directly or indirectly involved in the intermolecular contacts.
通过应用亲和色谱和阳离子交换色谱技术，从镰状细胞贫血患者的红细胞溶血产物中分离出体内糖化的Hb S成分。以这种方式分离出全溶血产物的总糖化部分（GHb）、Hb SIc、Hb So-糖化（主要由赖氨酰残基的ε-NH2基团糖基化形成）和Hb So-非糖化部分。GHb含有约33%的Hb SIc和42%的Hb So（Hb So-糖化），其余为糖化的Hb F和Hb A2。正如预期的那样，2,3-DPG的结合仅在Hb SIc中受到影响，而在Hb So-糖化中不受影响。Hb SIc和Hb So-糖化在浓缩磷酸盐溶液中的溶解度更高，并且比Hb So的非糖基化形式具有更高的最低胶凝浓度。这些效应被解释为是由于直接或间接参与分子间接触的特定位点的糖基化修饰所致。
