• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过质外体蛋白几丁质酶样 1 重新定位含有重复序列的真菌效应物可阻止其毒性。

Re-localization of a repeat-containing fungal effector by apoplastic protein Chitinase-like 1 blocks its toxicity.

机构信息

State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing, 210095, China.

Engineering Research Center of Ministry of Education for Cotton Germplasm Enhancement and Application, Nanjing Agricultural University, Nanjing, 210095, China.

出版信息

Nat Commun. 2024 Nov 22;15(1):10122. doi: 10.1038/s41467-024-54470-0.

DOI:10.1038/s41467-024-54470-0
PMID:39578470
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11584738/
Abstract

A fungal effector that is toxic to plant cells was identified in Verticillium dahliae. The effector contains a non-canonical Common in several Fungal Extracellular Membrane proteins (CFEM) domain, a tandem repeat region consisting of four 14-amino acid repeats rich in proline, and a C-terminal region, thus is designated V. dahliae tetrapeptide repeat protein (VdTRP). The membrane targeting of VdTRP is vital for its cell toxicity. CFEM mediates the membrane targeting and the tandem repeat region exerts the toxic function upon cell membrane. The chitinase-like 1 (CTL1), an essential apoplastic protein of cotton, can redirect VdTRP from cell membrane to apoplast. Transgenic cotton overexpressing CTL1 greatly enhances cotton resistance to V. dahliae without affecting cotton growth and development, implicating its potential application in breeding cotton with high wilt resistance. Our data demonstrates that genetic manipulation of effector target constitutes potential strategy for improving crop resistance to fungal pathogens.

摘要

在黄萎病菌中鉴定到一种对植物细胞有毒的真菌效应子。该效应子含有一个非典型的普通真菌细胞外膜蛋白 (CFEM) 结构域、一个由四个富含脯氨酸的 14 个氨基酸重复组成的串联重复区和一个 C 末端区，因此被命名为黄萎病菌四肽重复蛋白 (VdTRP)。VdTRP 的膜靶向对于其细胞毒性至关重要。CFEM 介导膜靶向，串联重复区在细胞膜上发挥毒性功能。几丁质酶样 1 (CTL1) 是棉花必需的质外体蛋白，它可以将 VdTRP 从细胞膜重新定向到质外体。过表达 CTL1 的转基因棉花显著增强了棉花对黄萎病菌的抗性，而不影响棉花的生长和发育，这暗示了其在培育高枯萎病抗性棉花方面的潜在应用。我们的数据表明，效应子靶标的遗传操作构成了提高作物对真菌病原体抗性的潜在策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d617/11584738/7b543036602a/41467_2024_54470_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d617/11584738/98e9d6abc9fc/41467_2024_54470_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d617/11584738/edd5d69d7240/41467_2024_54470_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d617/11584738/9e59c8045580/41467_2024_54470_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d617/11584738/15185f4c26c6/41467_2024_54470_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d617/11584738/4193611050fe/41467_2024_54470_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d617/11584738/ff22063fb817/41467_2024_54470_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d617/11584738/ac740dff1d9b/41467_2024_54470_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d617/11584738/1e8843d5643d/41467_2024_54470_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d617/11584738/7b543036602a/41467_2024_54470_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d617/11584738/98e9d6abc9fc/41467_2024_54470_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d617/11584738/edd5d69d7240/41467_2024_54470_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d617/11584738/9e59c8045580/41467_2024_54470_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d617/11584738/15185f4c26c6/41467_2024_54470_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d617/11584738/4193611050fe/41467_2024_54470_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d617/11584738/ff22063fb817/41467_2024_54470_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d617/11584738/ac740dff1d9b/41467_2024_54470_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d617/11584738/1e8843d5643d/41467_2024_54470_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d617/11584738/7b543036602a/41467_2024_54470_Fig9_HTML.jpg

相似文献

1
Re-localization of a repeat-containing fungal effector by apoplastic protein Chitinase-like 1 blocks its toxicity.通过质外体蛋白几丁质酶样 1 重新定位含有重复序列的真菌效应物可阻止其毒性。
Nat Commun. 2024 Nov 22;15(1):10122. doi: 10.1038/s41467-024-54470-0.
2
The Cotton Apoplastic Protein CRR1 Stabilizes Chitinase 28 to Facilitate Defense against the Fungal Pathogen .棉质体蛋白 CRR1 稳定几丁质酶 28 以促进对真菌病原体的防御。
Plant Cell. 2019 Feb;31(2):520-536. doi: 10.1105/tpc.18.00390. Epub 2019 Jan 16.
3
Participates in Cotton Resistance to Verticillium Wilt by Regulating Stigmasterol Synthesis and Plasma Membrane Stability.参与棉花抗黄萎病的机制是通过调节豆甾醇合成和质膜稳定性。
Int J Mol Sci. 2022 Jul 29;23(15):8437. doi: 10.3390/ijms23158437.
4
Cotton S-adenosylmethionine decarboxylase-mediated spermine biosynthesis is required for salicylic acid- and leucine-correlated signaling in the defense response to Verticillium dahliae.棉花S-腺苷甲硫氨酸脱羧酶介导的精胺生物合成是棉花对大丽轮枝菌防御反应中水杨酸和亮氨酸相关信号传导所必需的。
Planta. 2016 Apr;243(4):1023-39. doi: 10.1007/s00425-015-2463-5. Epub 2016 Jan 13.
5
Volatiles emitted by Pseudomonas aurantiaca ST-TJ4 trigger systemic plant resistance to Verticillium dahliae.橙色粘帚霉 ST-TJ4 挥发物诱导植物对黄萎病菌的系统抗性。
Microbiol Res. 2024 Oct;287:127834. doi: 10.1016/j.micres.2024.127834. Epub 2024 Jul 14.
6
Verticillium dahliae Elicitor VdSP8 Enhances Disease Resistance Through Increasing Lignin Biosynthesis in Cotton.大丽轮枝菌激发子VdSP8通过增加棉花中木质素的生物合成来增强抗病性。
Plant Cell Environ. 2025 Jan;48(1):728-745. doi: 10.1111/pce.15170. Epub 2024 Sep 26.
7
Salicylic acid-related cotton (Gossypium arboreum) ribosomal protein GaRPL18 contributes to resistance to Verticillium dahliae.水杨酸相关的棉花（亚洲棉）核糖体蛋白GaRPL18有助于抵抗大丽轮枝菌。
BMC Plant Biol. 2017 Mar 3;17(1):59. doi: 10.1186/s12870-017-1007-5.
8
GhMYB4 downregulates lignin biosynthesis and enhances cotton resistance to Verticillium dahliae.GhMYB4 下调木质素生物合成并增强棉花对黄萎病菌的抗性。
Plant Cell Rep. 2021 Apr;40(4):735-751. doi: 10.1007/s00299-021-02672-x. Epub 2021 Feb 27.
9
Positive roles of the Ca sensors GbCML45 and GbCML50 in improving cotton Verticillium wilt resistance.GbCML45 和 GbCML50 钙传感器在提高棉花黄萎病抗性中的积极作用。
Mol Plant Pathol. 2024 Jun;25(6):e13483. doi: 10.1111/mpp.13483.
10
Cotton SNARE complex component GhSYP121 regulates salicylic acid signaling during defense against Verticillium dahliae.棉花 SNARE 复合体组成蛋白 GhSYP121 调控对黄萎病菌（Verticillium dahliae）防御过程中的水杨酸信号。
J Cell Physiol. 2024 Nov;239(11):e31329. doi: 10.1002/jcp.31329. Epub 2024 May 27.

本文引用的文献

1
How plants manage pathogen infection.植物如何应对病原体感染。
EMBO Rep. 2024 Jan;25(1):31-44. doi: 10.1038/s44319-023-00023-3. Epub 2023 Dec 19.
2
Imaging Structural and Electrical Changes of Aging Cells Using Scanning Ion Conductance Microscopy.利用扫描离子电导显微镜观察衰老细胞的结构和电变化。
Small Methods. 2024 Aug;8(8):e2301315. doi: 10.1002/smtd.202301315. Epub 2023 Dec 10.
3
Improving rice nitrogen-use efficiency by modulating a novel monouniquitination machinery for optimal root plasticity response to nitrogen.
通过调控新型单泛素化机制提高水稻氮利用效率以优化根对氮的可塑性响应
Nat Plants. 2023 Nov;9(11):1902-1914. doi: 10.1038/s41477-023-01533-7. Epub 2023 Oct 5.
4
Address the growing urgency of fungal disease in crops.应对作物真菌病害日益紧迫的问题。
Nature. 2023 May;617(7959):31-34. doi: 10.1038/d41586-023-01465-4.
5
Cotton peroxisome-localized lysophospholipase counteracts the toxic effects of Verticillium dahliae NLP1 and confers wilt resistance.棉花过氧化物酶体定位的溶血磷脂酶能抵抗黄萎病菌 NLP1 的毒性作用并赋予其抗枯萎病的能力。
Plant J. 2023 Jul;115(2):452-469. doi: 10.1111/tpj.16236. Epub 2023 Apr 17.
6
Disarm resistance: Fungal effectors target WAK alternative splicing variant for virulence.消除抗性：真菌效应蛋白靶向WAK可变剪接变体以实现毒力。
Cell Rep. 2023 Jan 31;42(1):111939. doi: 10.1016/j.celrep.2022.111939. Epub 2023 Jan 4.
7
Fungal CFEM effectors negatively regulate a maize wall-associated kinase by interacting with its alternatively spliced variant to dampen resistance.真菌 CFEM 效应物通过与玉米壁相关激酶的可变剪接变体相互作用来负调控其活性，从而抑制抗性。
Cell Rep. 2022 Dec 27;41(13):111877. doi: 10.1016/j.celrep.2022.111877.
8
Recognition of glycoside hydrolase 12 proteins by the immune receptor RXEG1 confers Fusarium head blight resistance in wheat.免疫受体 RXEG1 识别糖苷水解酶 12 蛋白赋予小麦对赤霉病的抗性。
Plant Biotechnol J. 2023 Apr;21(4):769-781. doi: 10.1111/pbi.13995. Epub 2023 Jan 11.
9
ColabFold: making protein folding accessible to all.ColabFold：让蛋白质折叠变得人人可用。
Nat Methods. 2022 Jun;19(6):679-682. doi: 10.1038/s41592-022-01488-1. Epub 2022 May 30.
10
Evasion of plant immunity by microbial pathogens.微生物病原体对植物免疫的逃避。
Nat Rev Microbiol. 2022 Aug;20(8):449-464. doi: 10.1038/s41579-022-00710-3. Epub 2022 Mar 16.