Wang Xiaoli, Yang Huiru, Sun Tao, Zhang Jiale, Wang Lixuan, Zhang Yuting, Zhou Nandi
School of Biotechnology and Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi, 214122, China.
School of Biotechnology and Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi, 214122, China.
Biosens Bioelectron. 2025 Feb 15;270:116964. doi: 10.1016/j.bios.2024.116964. Epub 2024 Nov 19.
Mucin1 (MUC1) is a cell surface transmembrane protein overexpressed in multiple types of tumor cells, which is generally considered as a tumor-associated biomarker. Thus, quantifying and imaging of MUC1 in tumor cells is of great significance for the diagnosis and biological therapy of tumors. Herein, a fluorescence (FL) and surface-enhanced Raman scattering (SERS) dual-mode biosensor was developed for sensitive detection and imaging of MUC1 in living cells. The FL-SERS biosensor was based on self-assembled satellite structures mediated by the competition of MUC1 and complementary DNA modified on Au nanostars (AuNS-cDNA-PEG) with aptamer modified on quantum dots (QD-PDDA-Apt). This biosensor achieved dual-mode quantification and imaging of MUC1 by quenching the FL signal of QD and significantly enhancing the SERS signal of PDDA through the metal FL quenching effect and hotspot effect of AuNS, respectively. The dual-mode biosensor exhibited high sensitivity to MUC1, with a detection limit of 1.19 fg/mL under FL mode and 1.16 fg/mL under SERS mode. Moreover, this biosensor displayed good selectivity, nice biological stability and low cytotoxicity. Importantly, this biosensor possessed an excellent MUC1 dual-mode imaging capability with high specificity in different tumor cells, providing a new idea for clinical diagnosis of tumors.
粘蛋白1（MUC1）是一种在多种肿瘤细胞中过表达的细胞表面跨膜蛋白，通常被视为肿瘤相关生物标志物。因此，对肿瘤细胞中MUC1进行定量和成像对于肿瘤的诊断和生物治疗具有重要意义。在此，开发了一种荧光（FL）和表面增强拉曼散射（SERS）双模生物传感器，用于活细胞中MUC1的灵敏检测和成像。该FL-SERS生物传感器基于由修饰在金纳米星上的MUC1和互补DNA（AuNS-cDNA-PEG）与修饰在量子点上的适体（QD-PDDA-Apt）竞争介导的自组装卫星结构。该生物传感器分别通过金纳米星的金属荧光猝灭效应和热点效应猝灭量子点的荧光信号并显著增强聚二烯二甲基氯化铵的SERS信号，实现了MUC1的双模定量和成像。该双模生物传感器对MUC1表现出高灵敏度，在FL模式下检测限为1.19 fg/mL，在SERS模式下检测限为1.16 fg/mL。此外，该生物传感器具有良好的选择性﹑生物稳定性和低细胞毒性。重要的是，该生物传感器在不同肿瘤细胞中具有优异的MUC1双模成像能力和高特异性，为肿瘤的临床诊断提供了新思路。
