Yu Wenyan, Lin Xianfeng, Duan Nuo, Wang Zhouping, Wu Shijia
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, 214122, China.
Anal Chim Acta. 2023 Mar 1;1244:340846. doi: 10.1016/j.aca.2023.340846. Epub 2023 Jan 17.
Deoxynivalenol (DON), a common mycotoxin produced by Fusarium species, poses a great threat to human and animal body. Hence, it is of significance to develop an ultrasensitive and reliable method for DON detection. Herein, a fluorescence and surface-enhanced Raman scattering (FL-SERS) dual-mode aptasensor was designed for the detection of DON based on gold nanoclusters (Au NCs) and silver nanoparticles modified metal-polydopamine framework (Ag NPs/MPDA). In this aptasensor, complementary DNA modified Au NCs (cDNA-Au NCs) was selected as fluorescence probe, and 6-carboxytetramethylrhodamine (TAMRA)-labeled aptamer modified Ag NPs/MPDA (Ag NPs/MPDA-Apt-TAMRA) was employed as SERS probe, in which Ag NPs/MPDA acted as SERS substance and fluorescence quencher, and TAMRA acted as Raman label. The superior binding affinity of the aptamer with DON to cDNA can regulate the fluorescence and Raman signal intensities and realize the quantitative determination of DON. Under the optimal conditions, the aptasensor exhibited a low detection limit of 0.08 ng mL (0.1-100 ng mL) in FL mode and 0.06 ng mL (0.1-100 ng mL) in SERS mode. In addition, it was successfully applied for DON detection in wheat flour. We believe that the proposed FL-SERS strategy has a promising application in the detection of mycotoxins.
脱氧雪腐镰刀菌烯醇（DON）是镰刀菌属产生的一种常见霉菌毒素，对人体和动物体构成巨大威胁。因此，开发一种超灵敏且可靠的DON检测方法具有重要意义。在此，基于金纳米簇（Au NCs）和银纳米颗粒修饰的金属-聚多巴胺框架（Ag NPs/MPDA）设计了一种荧光和表面增强拉曼散射（FL-SERS）双模适体传感器用于检测DON。在这种适体传感器中，选择互补DNA修饰的Au NCs（cDNA-Au NCs）作为荧光探针，6-羧基四甲基罗丹明（TAMRA）标记的适体修饰的Ag NPs/MPDA（Ag NPs/MPDA-Apt-TAMRA）作为SERS探针，其中Ag NPs/MPDA作为SERS物质和荧光猝灭剂，TAMRA作为拉曼标记。适体与DON对cDNA的优异结合亲和力可调节荧光和拉曼信号强度，实现DON的定量测定。在最佳条件下，该适体传感器在FL模式下的检测限低至0.08 ng/mL（0.1 - 100 ng/mL），在SERS模式下为0.06 ng/mL（0.1 - 100 ng/mL）。此外，它成功应用于小麦粉中DON的检测。我们相信所提出的FL-SERS策略在霉菌毒素检测方面具有广阔的应用前景。
