Mahmoud Mahmoud A, Ibrahim Mustafa M
Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza PO 12211, Egypt.
Animal Health Research Institute, Dokki, Giza PO 12618, Egypt; Ministry of Environment, Water and Agriculture, Fish Welfare Branch, El-Jubail Province, Saudi Arabia.
Tissue Cell. 2024 Dec;91:102629. doi: 10.1016/j.tice.2024.102629. Epub 2024 Nov 18.
During routine veterinary inspection of fish from fishing boats, fish auction yards, and fish landing stations, as well as the large fish market for detection of fish diseases, abnormalities and/or overgrowth in body surfaces and evaluation of hygienic conditions and fish quality at El-Jubail Province, Saudi Arabia., external neoplastic overgrowths were observed in two fish species, giant sea catfish (Arius thalassinus) and Delagoa threadfin bream (Nemipterus bipunctatu). In both fishes, the neoplasms appeared as bony masses, and it was hard in its consistency. In the giant sea catfish (Arius thalassinus), the tumor appeared as three multifocal hard swellings at different locations (in the head region, at the dorsal fin, and near the caudal fin). The tumor mass in the head region of this fish was observed at the base of the right angle of the lower jaw, and it was hard, bony, nodular to round, and of 0.7 cm in diameter. The second one was observed at the dorsal fin and appeared as a small oval to rod hard mass of 0.5 cm in diameter, while the third mass appeared as a large and irregular wart-shaped bony swelling, about 3 cm in diameter, that extended laterally on the left aspect of the caudal peduncle near the caudal fin. In radiographic examination, the neoplasm appeared small, round to large, irregular, dense bony overgrowth with variable sizes. In histopathological examination, evidences of fibro-osteosarcoma associated with prominent multiple rounded eosinophilic apoptotic bodies in the neoplastic bony trabeculae were observed. On the other side, the Delagoa threadfin bream (Nemipterus bipunctatus) showed only one yellowish to reddish, pedunculated, irregular, and very hard swelling at the base of the median site of the dorsal fin, and it was firmly connected to the spine of the dorsal fin; it was about 1 cm high with a wide base of 0.5 cm. In histopathological examination, the bony neoplastic masses in this fish appeared with massive edematous fibrous connective tissue and newly formed blood capillaries; abnormal mitosis, hemorrhages, and hemosiderin pigment deposition in the central and peripheral parts of the neoplasm were observed. New bone formation was confirmed using histochemical staining. Our results indicated that these two species are vulnerable to fibro-osteosarcoma. Further environmental investigations as well as immunohistochemical and molecular studies are required to indicate the potential impact of the environmental pollution on the incidence of the neoplasms in this locality and to correlate the cellular evidence of the neoplasms to a specific fish species.
在对沙特阿拉伯朱拜勒省渔船、鱼类拍卖场、鱼货上岸站以及大型鱼类市场的鱼类进行常规兽医检查，以检测鱼病、体表异常和/或过度生长，并评估卫生状况和鱼的质量时，在两种鱼类中观察到了外部肿瘤性过度生长，即巨海鲶（Arius thalassinus）和德拉戈阿金线鲷（Nemipterus bipunctatu）。在这两种鱼中，肿瘤均表现为骨质肿块，质地坚硬。在巨海鲶（Arius thalassinus）中，肿瘤表现为不同部位的三个多灶性硬肿（头部区域、背鳍处和尾鳍附近）。该鱼头部区域的肿瘤位于下颌右角基部，坚硬、骨质、结节状至圆形，直径0.7厘米。第二个肿瘤在背鳍处观察到，表现为直径0.5厘米的小椭圆形至棒状硬肿块，而第三个肿块表现为直径约3厘米的大的不规则疣状骨质肿胀，在靠近尾鳍的尾柄左侧横向延伸。在射线照相检查中，肿瘤表现为小的、圆形至大的、不规则的、致密的骨质过度生长，大小不一。在组织病理学检查中，观察到纤维骨肉瘤的证据，肿瘤性骨小梁中有明显的多个圆形嗜酸性凋亡小体。另一方面，德拉戈阿金线鲷（Nemipterus bipunctatus）仅在背鳍中部基部出现一个淡黄色至红色、有蒂、不规则且非常硬的肿胀，它与背鳍的脊柱紧密相连；高约1厘米，基部宽0.5厘米。在组织病理学检查中，该鱼的骨质肿瘤性肿块表现为大量水肿性纤维结缔组织和新形成的血管；在肿瘤的中央和周边部分观察到异常有丝分裂、出血和含铁血黄素色素沉积。使用组织化学染色证实了新骨形成。我们的结果表明，这两个物种易患纤维骨肉瘤。需要进一步的环境调查以及免疫组织化学和分子研究，以表明环境污染对该地区肿瘤发病率的潜在影响，并将肿瘤的细胞证据与特定鱼类物种相关联。
