Liang Yu-Cheng, Cheng Yu-Shen, Chen Hsiao-Yi, Wang Hui-Min David
Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Yunlin, Taiwan, ROC; Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan, ROC.
Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Yunlin, Taiwan, ROC.
Int J Biol Macromol. 2025 Jan;284(Pt 1):137938. doi: 10.1016/j.ijbiomac.2024.137938. Epub 2024 Nov 22.
Wound adhesion after any abdominal and gastrointestinal surgery is as high as 90 %, and if the adhesion is severe, it will be fatal. During the operation, various methods are used to avoid the adhesion of organs, such as reducing surgical wound area and using hydrogel products with short gelation time. However, these gels might cause allergy, and the gels are unsuitable for long-lasting recovery processes because the body absorbs them too quickly to avoid sticking. Hence, we developed a long-lasting, temperature-sensitive hydrogel using arginyl-glycyl-aspartic acid to help heal postoperative wounds. The hydrogel formed at 37 °C, degraded in 7 days up to 50 % and provided a practicable anti-adhesion function. As the cell and animal tests indicated, the hydrogel exhibited preferable biocompatibility and was proven to effectively avoid adhesion behavior after abdominal surgery while boosting tissue proliferation; animal experiments have confirmed that hydrogel can effectively avoid adhesion after abdominal surgery and help tissue proliferation. This hydrogel inhibits SMAD, P38, and PI3K pathways by blocking fibrosis cytokines and TGF-β downstream signaling. This TGF-β inhibition promotes histoarchitecture remodeling and reduces cecum tissue fibrosis. Our hydrogel provides a new option for anti-sticking products and can be applied to versatile medical uses in the future.
任何腹部和胃肠道手术后的伤口粘连率高达90%，如果粘连严重，将是致命的。在手术过程中，人们使用各种方法来避免器官粘连，如减小手术伤口面积和使用凝胶化时间短的水凝胶产品。然而，这些凝胶可能会引起过敏，而且由于身体吸收它们太快而无法避免粘连，因此这些凝胶不适用于长期恢复过程。因此，我们使用精氨酰-甘氨酰-天冬氨酸开发了一种长效、温度敏感的水凝胶，以帮助愈合术后伤口。该水凝胶在37℃形成，7天内降解达50%，并提供了切实可行的抗粘连功能。正如细胞和动物试验所示，该水凝胶表现出较好的生物相容性，经证实能有效避免腹部手术后的粘连行为，同时促进组织增殖；动物实验已证实，水凝胶可有效避免腹部手术后的粘连并帮助组织增殖。这种水凝胶通过阻断纤维化细胞因子和TGF-β下游信号来抑制SMAD、P38和PI3K途径。这种对TGF-β的抑制促进组织结构重塑并减少盲肠组织纤维化。我们的水凝胶为抗粘连产品提供了一种新选择，未来可应用于多种医疗用途。
