Buyuksungur Senem, Tanir Tugba Endogan, Hasirci Vasif, Hasirci Nesrin
Center of Excellence in Biomaterials and Tissue Engineering (BIOMATEN), Middle East Technical University (METU), Ankara 06800, Türkiye.
Central Laboratory, Middle East Technical University (METU), Ankara 06800, Türkiye.
Biomacromolecules. 2025 May 12;26(5):3139-3154. doi: 10.1021/acs.biomac.5c00181. Epub 2025 Apr 29.
Poly(2-alkyl-2-oxazoline) (PAOx) polymers are promising materials due to their tunable properties. In this study, poly(2-ethyl-2-oxazoline) (PEtOx) was methacrylated after partial hydrolysis to produce methacrylated poly(2-ethyl-2-oxazoline) (POx-MA), which was subsequently used to synthesize novel hydrogels. Interpenetrating polymer networks (IPN) were developed by combining POx-MA with methacrylated gelatin (GelMA). Compression tests revealed that GelMA exhibited the highest mechanical strength (199 ± 21 kPa), followed by the IPN POx-MA:GelMA (112 ± 27 kPa) and POx-MA (15 ± 5 kPa). However, in scratch wound healing tests, this order was reversed, with POx-MA exhibiting the highest closure (67 ± 8%), followed by the IPN (51 ± 2%) and GelMA (42 ± 1%) in 48 h. Cell viability exceeded 90% with all of the hydrogels. The study showed that partial hydrolysis and the resultant free amine groups in POx-MA enhanced cell adhesion. Moreover, POx-MA containing hydrogels demonstrated high antibacterial activity against and . This study highlights the superior properties of POx-MA and POx-MA:GelMA IPN as novel hydrogels with substantial potential for biomaterials and tissue engineering applications.
聚（2-烷基-2-恶唑啉）（PAOx）聚合物因其可调谐性能而成为有前景的材料。在本研究中，聚（2-乙基-2-恶唑啉）（PEtOx）在部分水解后进行甲基丙烯酸酯化，以制备甲基丙烯酸酯化聚（2-乙基-2-恶唑啉）（POx-MA），随后用于合成新型水凝胶。通过将POx-MA与甲基丙烯酸酯化明胶（GelMA）结合来构建互穿聚合物网络（IPN）。压缩测试表明，GelMA表现出最高的机械强度（199±21 kPa），其次是IPN POx-MA:GelMA（112±27 kPa）和POx-MA（15±5 kPa）。然而，在划痕伤口愈合测试中，这个顺序颠倒了，POx-MA在48小时内表现出最高的闭合率（67±8%），其次是IPN（51±2%）和GelMA（42±1%）。所有水凝胶的细胞活力均超过90%。该研究表明，POx-MA中的部分水解和由此产生的游离胺基团增强了细胞粘附。此外，含POx-MA的水凝胶对[具体菌种1]和[具体菌种2]表现出高抗菌活性。本研究突出了POx-MA和POx-MA:GelMA IPN作为具有生物材料和组织工程应用巨大潜力的新型水凝胶的优异性能。
