Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei, 230012, China.
Key Laboratory of Medical Electronics and Digital Health of Zhejiang Province in Jiaxing University, College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, 314001, China.
Anal Chim Acta. 2024 Dec 15;1332:343353. doi: 10.1016/j.aca.2024.343353. Epub 2024 Oct 19.
As an antioxidant, SO plays a critical role in maintaining the plant's redox balance. At the same time, Hg as heavy metals, will accumulate in plants through soil, often disrupting physiological processes in plants. However, few studies have been reported to examine changes in Hg and SO in plants. Here, we developed a dual-channel fluorescent probe NYD, which can differentiate between the detection of Hg and SO.
The probe exhibits high sensitivity (LOD, 44 nM for Hg and 26 nM for SO). When Hg and HSO deprotect and attack the double bond by nucleophilic addition, significant emission of fluorescence at 475 nm and 574 nm with blue and red fluorescence respectively. In addition, NYD has the capability to detect the levels of Hg and SO in cells and alfalfa seedlings. Meanwhile, the findings indicated that alfalfa seedlings were exposed to Cd, which led to redox imbalance and increased endogenous SO production.
We anticipate that this research will contribute to a better understanding of the functions of SO under oxidative stress induced by Cd.
作为一种抗氧化剂，SO 在维持植物的氧化还原平衡中起着关键作用。与此同时，Hg 作为重金属，会通过土壤在植物中积累，常常扰乱植物的生理过程。然而，目前很少有研究报道检查植物中 Hg 和 SO 的变化。在这里，我们开发了一种双通道荧光探针 NYD，可以区分 Hg 和 SO 的检测。
该探针表现出高灵敏度（LOD，Hg 为 44 nM，SO 为 26 nM）。当 Hg 和 HSO 通过亲核加成脱保护并攻击双键时，分别以蓝色和红色荧光发射出 475nm 和 574nm 的显著荧光。此外，NYD 具有检测细胞和紫花苜蓿幼苗中 Hg 和 SO 水平的能力。同时，研究结果表明，Cd 暴露会导致苜蓿幼苗发生氧化还原失衡，内源 SO 产量增加。
我们预计，这项研究将有助于更好地理解 Cd 诱导的氧化应激下 SO 的功能。
