Institute of Innovation Materials and Energy, School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, China.
Laboratory of Engineering Profile, Satbayev University, Almaty, 050013, Kazakhstan.
Anal Chim Acta. 2024 Dec 15;1332:343354. doi: 10.1016/j.aca.2024.343354. Epub 2024 Oct 22.
BACKGROUND: Bisphenol A (BPA) has been identified as an endocrine disruptor with numerous detrimental effects on human health. There is an urgent need to develop fluorescence/colorimetric dual-mode sensing approaches with expanded detection linear range, increased accuracy, and enhanced application flexibility for BPA detection. The utilization of fluorescence and colorimetric signals in point-of-care applications and real-time sensitive sensing further highlights the significance of developing novel and efficient fluorescence/colorimetric dual-mode sensing platform with high-efficiency probes. RESULTS: Herein, a fluorescence and colorimetric dual-mode aptasensor was developed by using CsPbBr@Cu-MOF as the aptamer immobilization matrix and signal generator. CsPbBr was functionalized with Cu-MOF using a simple two-step strategy. This strategy involved in-situ formation and modified ligand-assisted precipitation technique with 4,4'-bipyridine (4,4-Bpy) serving as the bifunctional linker. The resulting CsPbBr@Cu-MOF exhibited improved stability in water and enhanced fluorescence. Additionally, it functioned as peroxidase mimetic to oxidize 3,3',5,5'-tetramethyl benzidine (TMB), leading to a colorimetric change from colorless to blue. In the presence of BPA, aptamers were removed from CsPbBr@Cu-MOF. Consequently, the fluorescence and peroxidase-activity of CsPbBr@Cu-MOF were recovered, resulting in the enhanced fluorescence intensity and color change of TMB. Using this system, the proposed aptasensor demonstrated detection ranges of 1.0-80.0 nM with a LOD of 0.60 nM for the colorimetric method, and a linearity range of 0.1-100 nM with a LOD of 0.02 nM for the fluorescence method. SIGNIFICANCE: The obtained CsPbBr@Cu-MOF composites showed excellent fluorescence properties, good peroxidase-like activity, and aqueous stability. Furthermore, the proposed dual-mode aptasensor demonstrated simplicity, cost-effectiveness and good anti-interference abilities. This can be extended to the construction of other dual-mode sensors by changing aptamers and provides novel insights on the potential applications of perovskites in bioanalysis.
背景：双酚 A（BPA）已被确定为一种内分泌干扰物，对人类健康有许多不利影响。因此，迫切需要开发具有扩展检测线性范围、提高准确性和增强应用灵活性的荧光/比色双模式传感方法，用于 BPA 检测。荧光和比色信号在即时检测应用和实时敏感传感中的利用进一步凸显了开发具有高效探针的新型和高效荧光/比色双模式传感平台的重要性。
结果：本文采用 CsPbBr@Cu-MOF 作为适体固定基质和信号发生器，构建了一种荧光和比色双模式适体传感器。通过简单的两步策略，将 CsPbBr 功能化接枝到 Cu-MOF 上。该策略涉及原位形成和修饰配体辅助沉淀技术，其中 4,4'-联吡啶（4,4-Bpy）作为双功能连接体。所得的 CsPbBr@Cu-MOF 在水中表现出增强的稳定性和荧光增强，并且还可以作为过氧化物酶模拟物氧化 3,3',5,5'-四甲基联苯胺（TMB），导致 TMB 从无色到蓝色的比色变化。在存在 BPA 的情况下，适体从 CsPbBr@Cu-MOF 上被去除。因此，CsPbBr@Cu-MOF 的荧光和过氧化物酶活性得到恢复，导致 TMB 的荧光增强和颜色变化。使用该系统，所提出的适体传感器在比色法中具有 1.0-80.0 nM 的检测范围和 0.60 nM 的检测限，在荧光法中具有 0.1-100 nM 的线性范围和 0.02 nM 的检测限。
意义：所获得的 CsPbBr@Cu-MOF 复合材料表现出优异的荧光性能、良好的过氧化物酶样活性和水稳定性。此外，所提出的双模式适体传感器具有简单、经济高效和良好的抗干扰能力。通过改变适体，这种方法可以扩展到其他双模式传感器的构建，并为钙钛矿在生物分析中的潜在应用提供新的见解。
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