Liao Song, Gui Liuyang, Yang Yufan, Liu Yiwei, Hu Xiaopeng
College of Life Science, Yangtze University, Jingzhou, 434023, Hubei Province, People's Republic of China.
Biogas Institute of Ministry of Agriculture and Rural Affairs, Chengdu, 610041, Sichuan Province, People's Republic of China.
Mikrochim Acta. 2024 Jul 31;191(8):497. doi: 10.1007/s00604-024-06579-8.
A dual-mode fluorescence/visual aptasensor was developed for straightforward and accurate determination of aflatoxin B1 (AFB1) based on an Au/metal-organic framework (Au/MOF) composite. Aptamer-modified Au/FeO (Apt/Au/FeO) served as the recognition element, and Au/MOF modified with complementary chains and 3,3',5,5'-tetramethylbenzidine (cDNA/TMB/Au/MOF) acted as the fluorescence and visual probes. These components are integrated to form conjugates (Apt/Au/FeO-cDNA/TMB/Au/MOF). Upon the introduction of AFB1, some cDNA/TMB/Au/MOF dissociated from Apt/Au/FeO, enabling the use of detached probes for visual detection. The undecomposed conjugates were isolated magnetically for use in fluorescence detection. As the AFB1 concentration increases, the visual signal intensifies and fluorescence intensity diminishes. Thus, the proposed aptasensor achieves the simultaneous fluorescence and visual determination of AFB1, obviating the need for material and reagent substitutions. The detection limits were established at 0.07 ng mL for the fluorescence mode and 0.08 ng mL for the visual mode. The effectiveness of the aptasensor was further validated by quantifying AFB1 in real samples.
基于金/金属有机框架（Au/MOF）复合材料，开发了一种双模式荧光/可视化适体传感器，用于直接、准确地测定黄曲霉毒素B1（AFB1）。适配体修饰的金/氧化亚铁（Apt/Au/FeO）用作识别元件，用互补链和3,3',5,5'-四甲基联苯胺修饰的Au/MOF（cDNA/TMB/Au/MOF）作为荧光和可视化探针。这些组分整合形成缀合物（Apt/Au/FeO-cDNA/TMB/Au/MOF）。引入AFB1后，一些cDNA/TMB/Au/MOF从Apt/Au/FeO上解离，使得能够使用分离的探针进行可视化检测。未分解的缀合物通过磁性分离用于荧光检测。随着AFB1浓度增加，可视化信号增强而荧光强度减弱。因此，所提出的适体传感器实现了AFB1的荧光和可视化同时测定，无需更换材料和试剂。荧光模式下的检测限为0.07 ng/mL，可视化模式下的检测限为0.08 ng/mL。通过对实际样品中的AFB1进行定量，进一步验证了适体传感器的有效性。
