用于分离牙龈拟杆菌的选择性培养基。
Selective medium for the isolation of Bacteroides gingivalis.
作者信息
Hunt D E, Jones J V, Dowell V R
出版信息
J Clin Microbiol. 1986 Mar;23(3):441-5. doi: 10.1128/jcm.23.3.441-445.1986.
Abstract
Bacteroides gingivalis has been implicated in various forms of periodontal disease and may be responsible for other diseases in humans. The role of B. gingivalis in disease has been difficult to assess, because it is inhibited by most selective media commonly used by clinical laboratories to aid in isolating gram-negative, nonsporeforming anaerobes. We have developed a new medium, Bacteroides gingivalis agar, which contains bacitracin, colistin, and nalidixic acid as selective agents. This medium allowed B. gingivalis to be isolated from oral specimens with little difficulty and also allowed B. gingivalis to be isolated from phenotypically similar Bacteroides species, such as B. asaccharolyticus and B. endodontalis, with which it can easily be confused.
摘要
牙龈拟杆菌与多种形式的牙周疾病有关，可能也与人类的其他疾病有关。牙龈拟杆菌在疾病中的作用一直难以评估，因为它会被临床实验室常用的大多数用于辅助分离革兰氏阴性、无芽孢厌氧菌的选择性培养基抑制。我们开发了一种新的培养基——牙龈拟杆菌琼脂，它含有杆菌肽、黏菌素和萘啶酸作为选择性试剂。这种培养基能让牙龈拟杆菌很容易地从口腔标本中分离出来，还能让牙龈拟杆菌从表型相似的拟杆菌属物种（如解糖拟杆菌和牙髓拟杆菌）中分离出来，而牙龈拟杆菌很容易与这些物种混淆。
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