Leathers Tess A, Ramarapu Raneesh, Rogers Crystal D
Department of Anatomy, Physiology, and Cell Biology, University of California, Davis, School of Veterinary Medicine, Davis, CA, USA.
Department of Anatomy, Physiology, and Cell Biology, University of California, Davis, School of Veterinary Medicine, Davis, CA, USA.
Dev Biol. 2025 Feb;518:61-70. doi: 10.1016/j.ydbio.2024.11.009. Epub 2024 Nov 22.
Vertebrate development is regulated by several complex well-characterized morphogen signaling pathways, transcription factors, and structural proteins, but less is known about the enzymatic pathways that regulate early development. We have identified that factors from the inflammation-mediating cyclooxygenase (COX) signaling pathway are expressed at early stages of development in avian embryos. Using Gallus gallus (chicken) as a research model, we characterized the spatiotemporal expression of a subset of genes and proteins in the COX pathway during early neural development stages. Specifically, here we show expression patterns of COX-1, COX-2, and microsomal prostaglandin E synthase-2 (mPGES-2) as well as the genes encoding these enzymes (PTGS1, PTGS2, and PTGES-2). Unique expression patterns of individual players within the COX pathway suggest that they may play non-canonical/non-traditional roles in the embryo compared to their roles in the adult. Future work should examine the function of the COX pathway in tissue specification and morphogenesis and determine if these expression patterns are conserved across species.
脊椎动物的发育受多种复杂且已被充分表征的形态发生素信号通路、转录因子和结构蛋白的调控，但对于调控早期发育的酶促途径了解较少。我们已经确定，炎症介导的环氧化酶（COX）信号通路中的因子在禽类胚胎发育的早期阶段就有表达。以原鸡（鸡）作为研究模型，我们对早期神经发育阶段COX途径中一组基因和蛋白质的时空表达进行了表征。具体而言，我们在此展示了COX-1、COX-2和微粒体前列腺素E合酶-2（mPGES-2）以及编码这些酶的基因（PTGS1、PTGS2和PTGES-2）的表达模式。COX途径中各个成员独特的表达模式表明，与它们在成体中的作用相比，它们可能在胚胎中发挥非经典/非传统的作用。未来的工作应该研究COX途径在组织特化和形态发生中的功能，并确定这些表达模式是否在物种间保守。
