Lu Jia-Bao, Ren Peng-Peng, Tian Ying, Yang Yan-Yan, Feng Qing-Kai, Zhang Xiao-Ya, He Fang, Huang Hai-Jian, Chen Jian-Ping, Li Jun-Min, Zhang Chuan-Xi
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, 315211, China.
Institute of Insect Science, Zhejiang University, Hangzhou, 310058, China.
Insect Biochem Mol Biol. 2025 Jan;176:104220. doi: 10.1016/j.ibmb.2024.104220. Epub 2024 Nov 22.
Insect oviposition secretions play crucial roles during the reproductive process, yet systematic studies on their structural characterization and protein compositions remain limited. This study investigated the oviposition secretions of three major rice pests: the brown planthopper (Nilaparvata lugens, BPH), small brown planthopper (Laodelphax striatella, SBPH), and white-backed planthopper (Sogatella furcifera, WBPH). Ultrastructural observation revealed differences in the oviposition secretions of them. The eggs of BPH and SBPH were adhered to rice tissue by abundant secretions, while WBPH eggs were embedded deeper within the leaf sheath with less secretions. Proteomic analysis identified 111, 98, and 66 oviposition secretion proteins (OSPs) in BPH, SBPH, and WBPH, respectively. 4 common protein subgroups were shared among them, along with varying numbers of shared subgroups between species pairs. Notably, the majority of OSPs were exclusively found in one species, indicating the existence of both similar and specialized functions unique to each planthopper species. The functions of 4 uncharacterized OSPs (Nl.chr07.0363, Nl.chr12.078, Nl.chr11.716, Nl.scaffold.0714) that were uniquely identified in the BPH were studied by maternal RNAi. Downregulation of each of these 4 protein-coding genes led to a significant decrease in egg production and hatchability. Moreover, knockdown of Nl.chr12.078 or Nl.chr07.0363 also disrupt the secretory function of the lateral oviduct. In conclusion, this study provides insights into the structural characteristics and protein components of the oviposition secretions of BPH, SBPH, and WBPH, which could serve as potential targets for RNAi-based pest control and lay a foundation for future studies on insect-plant interactions mediated by oviposition secretions.
昆虫产卵分泌物在生殖过程中起着至关重要的作用，然而对其结构特征和蛋白质组成的系统研究仍然有限。本研究调查了三种主要水稻害虫的产卵分泌物：褐飞虱（Nilaparvata lugens，BPH）、灰飞虱（Laodelphax striatella，SBPH）和白背飞虱（Sogatella furcifera，WBPH）。超微结构观察揭示了它们产卵分泌物的差异。BPH和SBPH的卵通过大量分泌物附着在水稻组织上，而WBPH的卵则以较少的分泌物更深地嵌入叶鞘内。蛋白质组学分析分别在BPH、SBPH和WBPH中鉴定出111、98和66种产卵分泌蛋白（OSP）。它们共有4个常见的蛋白质亚组，不同物种对之间还存在数量不等的共享亚组。值得注意的是，大多数OSP仅在一个物种中发现，这表明每种飞虱物种都存在相似和特有的功能。通过母体RNA干扰研究了在BPH中独特鉴定出的4种未表征的OSP（Nl.chr07.0363、Nl.chr12.078、Nl.chr11.716、Nl.scaffold.0714）的功能。这4个蛋白质编码基因中的每一个基因的下调都导致产卵量和孵化率显著下降。此外，敲低Nl.chr12.078或Nl.chr07.0363也会破坏侧输卵管的分泌功能。总之，本研究深入了解了BPH、SBPH和WBPH产卵分泌物的结构特征和蛋白质成分，这些成分可作为基于RNA干扰的害虫防治的潜在靶点，并为未来关于产卵分泌物介导的昆虫 - 植物相互作用的研究奠定基础。
