State Key Laboratory of Rice Biology & Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University , Hangzhou , China.
National Engineering Laboratory of Wheat and Maize & Ministry of Agricultural and Rural Affairs Key Laboratory of Biology and Genetic Improvement of Maize in Northern Yellow-huai River Plain, Maize Research Institute, Shandong Academy of Agricultural Sciences , Jinan , China.
GM Crops Food. 2019;10(3):170-180. doi: 10.1080/21645698.2019.1649530. Epub 2019 Jul 31.
Interspecific interactions are complex in agro-ecosystems and could be affected by agricultural technologies including transgenic crop planting. Few studies focused on the effects of Bt crops on the interspecific interactions of non-target organisms. Here we assessed the effects of transgenic rice (Cry2A rice) on the interspecific interaction between two rice planthoppers, namely, (the brown planthopper, BPH) and (the white-backed planthopper, WBPH). Cry2A rice showed no significant effects on most biological parameters of these two rice planthoppers, except for wet weight of BPH female adults and development duration of WBPH female nymphs. In contrast, interspecific interactions between BPH and WBPH showed significant impacts on their biological parameters, no matter on Cry2A rice or non-transgenic control. In two-factor analysis combing rice line and interspecific interaction together, the interaction between these two factors did not affect most biological parameters of neither planthopper species, except for development duration of BPH female nymphs and WBPH nymphs (both male and female). Additionally, the egg distributions of BPH and WBPH had no significant differences between Cry2A and non-Cry2A treatments. Results of field experiments showed that Cry2A rice did not affect their population densities at most sampling dates in a five-year survey, and the interaction between BPH and WBPH showed no significant differences in both Cry2A and non-Cry2A rice paddies. In conclusion, our tested Cry2A rice would not affect the interspecific interactions between BPH and WBPH based both laboratory and field results.
种间相互作用在农业生态系统中较为复杂，可能会受到包括转基因作物种植在内的农业技术的影响。很少有研究关注转基因作物对非靶标生物种间相互作用的影响。在这里，我们评估了转 Cry2A 基因水稻对两种水稻稻飞虱（褐飞虱和白背飞虱）种间相互作用的影响。Cry2A 水稻对这两种稻飞虱的大多数生物学参数没有显著影响，除了褐飞虱雌成虫的湿重和白背飞虱雌若虫的发育时间。相比之下，褐飞虱和白背飞虱之间的种间相互作用对它们的生物学参数有显著影响，无论是在 Cry2A 水稻还是非转基因对照中。在结合水稻品种和种间相互作用的两因素分析中，这两个因素之间的相互作用对这两个飞虱物种的大多数生物学参数没有影响，除了褐飞虱雌若虫和白背飞虱若虫（雄性和雌性）的发育时间。此外，Cry2A 和非 Cry2A 处理之间，褐飞虱和白背飞虱的卵分布没有显著差异。田间实验结果表明，在五年的调查中，Cry2A 水稻在大多数采样日期都不会影响其种群密度，并且在 Cry2A 和非 Cry2A 稻田中，褐飞虱和白背飞虱之间的相互作用没有显著差异。总之，根据实验室和田间结果，我们测试的 Cry2A 水稻不会影响褐飞虱和白背飞虱之间的种间相互作用。
