Department of Biotechnology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, 81746-73441, Iran.
Department of Chemistry, University of Isfahan, Isfahan, 81746-73441, Iran.
Sci Rep. 2024 Nov 24;14(1):29111. doi: 10.1038/s41598-024-80531-x.
A simple, one-pot and green method is reported for hydrothermal synthesis of highly fluorescent and magnetic carbon dots (CDs) by using D-glucose, as the carbon source. CDs were fully characterized by the UV-Vis and fluorescence spectroscopy, DLS, FTIR, TEM, EDS, XRD, and VSM. The nitrogen doping of different diamines significantly improved the fluorescence quantum yield (QY) of CDs with the maximum effect obtained by using m-phenylenediamine (mPDA). Temperature and reaction time also affected the QY of CDs with the best results obtained at 150 °C for 3 h. The heteroatom doping by innovative use of different metal sulfates including FeSO, MnSO, CuSO, MgSO, and ZnSO, further improved the optical properties of CDs. Interestingly, the magnetic and multicolor CDs with high colloidal stability and QYs of 17.7, 16.5, and 53.9% at 460, 490, and 515 nm, respectively, were synthesized by using 0.1 M of glucose, mPDA and MnSO. The resulted Mn-, S-, N-doped CDs represented rapid uptake and high-quality fluorescence imaging of the human fibroblast and umbilical vein endothelial cells in vitro, without significant toxicity. The CDs also displayed high r relaxivity of 32.3 mM s and were used for high-contrast MR and fluorescence imaging of mouse tumor models, in vivo.
本文报道了一种简单、一锅法和绿色的水热合成方法，以 D-葡萄糖为碳源，合成具有高荧光和磁性的碳点（CDs）。通过紫外可见光谱和荧光光谱、DLS、FTIR、TEM、EDS、XRD 和 VSM 对 CDs 进行了全面表征。不同二胺的氮掺杂显著提高了 CDs 的荧光量子产率（QY），其中使用间苯二胺（mPDA）的效果最佳。温度和反应时间也影响了 CDs 的 QY，最佳结果是在 150°C 下反应 3 小时。创新地使用不同的金属硫酸盐（包括 FeSO、MnSO、CuSO、MgSO 和 ZnSO）进行杂原子掺杂，进一步提高了 CDs 的光学性能。有趣的是，通过使用 0.1 M 的葡萄糖、mPDA 和 MnSO，合成了具有高胶体稳定性和分别为 17.7%、16.5%和 53.9%的 460、490 和 515nm 处量子产率的磁性和多色 CDs。所得的 Mn、S、N 掺杂 CDs 表现出对人成纤维细胞和脐静脉内皮细胞的快速摄取和高质量荧光成像，且没有明显的毒性。CDs 还表现出高 r2 弛豫率 32.3 mM s-1，并用于体内小鼠肿瘤模型的高对比度磁共振和荧光成像。
