Inan Oya Eralp, Levent Pinar, Saril Ahmet, Hamabe Lina, Kocaturk Meric, Yilmaz Zeki
Department of Animal Science, Faculty of Agriculture, Eskisehir Osmangazi Univesity, Eskisehir, Turkey.
Department of Internal Medicine, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa, Turkey.
Vet Med (Praha). 2024 Oct 30;69(10):345-354. doi: 10.17221/49/2024-VETMED. eCollection 2024 Oct.
Canine parvoviral enteritis (CPE) has a high mortality rate in untreated dogs due to systemic inflammation and multi-organ dysfunction. The inflammatory process can lead to coagulation abnormalities. This study aimed to evaluate the coagulation status using thromboelastography (TEG) and assess the thrombin generation (TG) and clot dissolution using TEG-derived velocity curve (v-curve) parameters in dogs with CPE. It included 21 dogs with CPE and five healthy dogs. In addition to the clinico-haemato-biochemical examinations, the coagulation status was analysed using citrated venous blood samples with TEG. All the dogs with CPE met at least two criteria for systemic inflammatory response syndrome (SIRS). The comparison to healthy controls showed a statistically significant prolongation of reaction times (R time; = 0.005) and times to the maximum rate of thrombus generation (TMRTG; = 0.003). However, the times to the maximum rate of lysis (TMRL; = 0.041) and total lysis (L; = 0.024) decreased significantly. The TEG tracings showed coagulation states varying from hypocoagulation to hypercoagulation in dogs with CPE. These results showed that the v-curve derivate can be used to evaluate the coagulation in dogs with CPE, and it could be superior to the standard TEG variables for determining the low fibrinolytic activity. Thus, the v-curve parameters may provide a novel insight into the underlying mechanism and clinical treatment strategy of CPE-induced inflammation.
犬细小病毒肠炎（CPE）在未经治疗的犬中死亡率很高，原因是全身炎症和多器官功能障碍。炎症过程可导致凝血异常。本研究旨在使用血栓弹力图（TEG）评估凝血状态，并使用TEG衍生的速度曲线（v曲线）参数评估CPE犬的凝血酶生成（TG）和凝块溶解情况。研究纳入了21只患有CPE的犬和5只健康犬。除临床血液生化检查外，还使用枸橼酸盐抗凝的静脉血样本通过TEG分析凝血状态。所有患有CPE的犬均至少符合两项全身炎症反应综合征（SIRS）标准。与健康对照组相比，反应时间（R时间；P = 0.005）和达到血栓生成最大速率的时间（TMRTG；P = 0.003）在统计学上有显著延长。然而，达到最大溶解速率的时间（TMRL；P = 0.041）和总溶解率（L；P = 0.024）显著降低。TEG描记图显示，CPE犬的凝血状态从低凝到高凝不等。这些结果表明，v曲线导数可用于评估CPE犬的凝血情况，在确定低纤溶活性方面可能优于标准TEG变量。因此，v曲线参数可能为CPE诱导的炎症的潜在机制和临床治疗策略提供新的见解。
