Nunes Aline, Dutra Felipe de Souza, Brito Sinara de Nazaré Santana, Pereira-Vasques Milene Stefani, Azevedo Gadiel Zilto, Schneider Alex Ricardo, Oliveira Eva Regina, Santos Alex Alves Dos, Maraschin Marcelo, Vianello Fábio, Lima Giuseppina Pace Pereira
Plant Biotechnology and Postharvest Laboratory, Department of Chemical and Biological Sciences, Institute of Biosciences, São Paulo State University, Botucatu 18618-970, SP, Brazil.
Laboratory of Biotechnology of Natural and Synthetics Products, Institute of Biotecnology, University of Caxias do Sul, Caxias do Sul 95070-560, RS, Brazil.
Methods Protoc. 2024 Nov 1;7(6):88. doi: 10.3390/mps7060088.
is a red seaweed used globally in various biotechnological processes. To ensure the content and stability of its bioactive compounds postharvest, suitable drying protocols must be adopted to provide high-quality raw materials for industrial use. This study aimed to analyze the influence of freeze-drying and oven-drying on the total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (FRAP and DPPH assays), total carotenoid content (TC), and lipase (LA) and protease activity (PA) of samples collected over the seasons in sea farms in southern Brazil. The freeze-drying technique was found to be more effective regarding superior contents of TPC (39.23 to 127.74 mg GAE/100 g) and TC (10.27 to 75.33 μg/g), as well as DPPH (6.12 to 8.91 mg/100 g). In turn, oven-drying proved to be the best method regarding the TFC (4.99 to 12.29 mg QE/100 g) and PA (119.50 to 1485.09 U/g), with better performance in the FRAP (0.28 to 0.70 mmol/100 g). In this way, it appears that the drying process of the algal biomass can be selected depending on the required traits of the biomass for the intended industrial application. In terms of cost-effectiveness, drying the biomass using oven-drying can be considered appropriate.
是一种在全球各种生物技术过程中使用的红海藻。为确保收获后其生物活性化合物的含量和稳定性，必须采用合适的干燥方案，以提供用于工业用途的高质量原材料。本研究旨在分析冷冻干燥和烘干对巴西南部海域养殖场不同季节采集的样本的总酚含量（TPC）、总黄酮含量（TFC）、抗氧化活性（FRAP和DPPH测定）、总类胡萝卜素含量（TC）以及脂肪酶（LA）和蛋白酶活性（PA）的影响。结果发现，冷冻干燥技术在TPC（39.23至127.74毫克GAE/100克）和TC（10.27至75.33微克/克）以及DPPH（6.12至8.91毫克/100克）含量较高方面更有效。反过来，烘干被证明是TFC（4.99至12.29毫克QE/100克）和PA（119.50至1485.09单位/克）方面的最佳方法，在FRAP（0.28至0.70毫摩尔/100克）方面表现更好。这样看来，藻类生物质的干燥过程可以根据预期工业应用中生物质所需的特性来选择。在成本效益方面，使用烘干干燥生物质可以被认为是合适的。
