Kholodenko I V, Lupatov A Y, Kim Y S, Saryglar R Y, Kholodenko R V, Yarygin K N
Institute of Biomedical Chemistry, Moscow, Russia.
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Bull Exp Biol Med. 2024 Nov;178(1):122-129. doi: 10.1007/s10517-024-06294-7. Epub 2024 Nov 25.
Screening of cell surface markers of three glioma cell lines (astrocytoma 1321N1, glioblastoma T98g, and glioblastoma astrocytoma U373 MG) was performed. Glioma cells expressed common mesenchymal cell markers, although the expression levels varied between the cell lines. The expression of proneural markers and glioma cancer stem cell markers was very low and also varied. Induction of differentiation towards the mesodermal cell lineages showed effective adipogenic and osteogenic differentiation for only the U373 MG cell line, while the 1321N1 and T98g lines demonstrated weak adipogenic potential and failed to undergo osteogenic differentiation. The obtained results point to the intratumor phenotypical heterogeneity of cells in gliomas and to the differences between the three studied types of gliomas with regard to the content of cells with mesenchymal phenotype.
对三种胶质瘤细胞系（星形细胞瘤1321N1、胶质母细胞瘤T98g和胶质母细胞瘤星形细胞瘤U373 MG）的细胞表面标志物进行了筛选。胶质瘤细胞表达常见的间充质细胞标志物，尽管各细胞系之间的表达水平有所不同。神经前体细胞标志物和胶质瘤癌干细胞标志物的表达非常低，且也存在差异。向中胚层细胞谱系的诱导分化仅对U373 MG细胞系显示出有效的脂肪生成和成骨分化，而1321N1和T98g细胞系显示出较弱的脂肪生成潜力，且未能进行成骨分化。所获得的结果表明胶质瘤中细胞的肿瘤内表型异质性，以及三种研究类型的胶质瘤在间充质表型细胞含量方面的差异。
