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用于分批和流动生物催化过程的固定化酶开发的进展与挑战

Advances and Challenges in the Development of Immobilized Enzymes for Batch and Flow Biocatalyzed Processes.

作者信息

Patti Stefania, Magrini Alunno Ilaria, Pedroni Sara, Riva Sergio, Ferrandi Erica Elisa, Monti Daniela

机构信息

Istituto di Scienze e Tecnologie Chimiche "G. Natta" (SCITEC), CNR, Via Bianco 9, 20131, Milano, Italy.

Department of Pharmaceutical Sciences, University of, Milan, Via Mangiagalli 25, 20133, Milano, Italy.

出版信息

ChemSusChem. 2025 Apr 14;18(8):e202402007. doi: 10.1002/cssc.202402007. Epub 2024 Dec 3.

DOI:10.1002/cssc.202402007
PMID:39585729
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11997919/
Abstract

The development of immobilized enzymes both for batch and continuous flow biocatalytic processes has gained significant traction in recent years, driven by the need for cost-effective and sustainable production methods in the fine chemicals and pharmaceutical industries. Enzyme immobilization not only enables the recycling of biocatalysts but also streamlines downstream processing, significantly reducing the cost and environmental impact of biotransformations. This review explores recent advancements in enzyme immobilization techniques, covering both carrier-free methods, entrapment strategies and support-based approaches. At this regard, the selection of suitable materials for enzyme immobilization is examined, highlighting the advantages and challenges associated with inorganic, natural, and synthetic organic carriers. Novel opportunities coming from innovative binding strategies, such as genetic fusion technologies, for the preparation of heterogeneous biocatalysts with enhanced activity and stability will be discussed as well. This review underscores the need for ongoing research to address current limitations and optimize immobilization strategies for industrial applications.

摘要

近年来，由于精细化工和制药行业对经济高效且可持续的生产方法的需求，用于间歇和连续流动生物催化过程的固定化酶的开发受到了广泛关注。酶固定化不仅能够实现生物催化剂的循环利用，还简化了下游处理过程，显著降低了生物转化的成本和环境影响。本综述探讨了酶固定化技术的最新进展，涵盖了无载体方法、包埋策略和基于载体的方法。在此方面，研究了用于酶固定化的合适材料的选择，突出了与无机、天然和合成有机载体相关的优点和挑战。还将讨论来自创新结合策略（如基因融合技术）的新机遇，这些策略用于制备具有更高活性和稳定性的非均相生物催化剂。本综述强调了持续研究以解决当前局限性并优化工业应用固定化策略的必要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc50/11997919/f08b13e7034e/CSSC-18-e202402007-g013.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc50/11997919/80622ea33e17/CSSC-18-e202402007-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc50/11997919/714c21de4235/CSSC-18-e202402007-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc50/11997919/203794c6226c/CSSC-18-e202402007-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc50/11997919/234e162b098e/CSSC-18-e202402007-g006.jpg
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