Helmholtz-Zentrum Hereon, Institute of Membrane Research, Max-Planck-Straße 1, 21502, Geesthacht, Germany.
RWTH Aachen University, Institute of Biotechnology, Worringerweg 3, 52074, Aachen, Germany.
Nat Commun. 2024 Apr 17;15(1):3308. doi: 10.1038/s41467-024-47007-y.
Continuous-flow biocatalysis utilizing immobilized enzymes emerged as a sustainable route for chemical synthesis. However, inadequate biocatalytic efficiency from current flow reactors, caused by non-productive enzyme immobilization or enzyme-carrier mismatches in size, hampers its widespread application. Here, we demonstrate a general-applicable and robust approach for the fabrication of a high-performance enzymatic continuous-flow reactor via integrating well-designed scalable isoporous block copolymer (BCP) membranes as carriers with an oriented and productive immobilization employing material binding peptides (MBP). Densely packed uniform enzyme-matched nanochannels of well-designed BCP membranes endow the desired nanoconfined environments towards a productive immobilized phytase. Tuning nanochannel properties can further regulate the complex reaction process and fortify the catalytic performance. The synergistic design of enzyme-matched carriers and efficient enzyme immobilization empowers an excellent catalytic performance with >1 month operational stability, superior productivity, and a high space-time yield (1.05 × 10 g L d) via a single-pass continuous-flow process. The obtained performance makes the designed nano- and isoporous block copolymer membrane reactor highly attractive for industrial applications.
利用固定化酶的连续流生物催化已成为化学合成的可持续途径。然而，当前的流动反应器由于非生产性的酶固定化或酶-载体在大小上不匹配，导致生物催化效率不足，阻碍了其广泛应用。在这里，我们展示了一种通用且稳健的方法，通过将设计良好的可扩展的具有各向异性和生产性固定化的介孔嵌段共聚物（BCP）膜作为载体与材料结合肽（MBP）整合在一起，制造高性能的酶连续流反应器。设计良好的 BCP 膜的密集均匀的酶匹配纳米通道提供了所需的纳米限域环境，有利于生产性固定化植酸酶。调整纳米通道的性质可以进一步调节复杂的反应过程并增强催化性能。酶匹配载体和高效酶固定化的协同设计通过单通道连续流工艺实现了优异的催化性能，具有超过 1 个月的操作稳定性、更高的生产力和高时空收率（1.05×10 g L d）。所获得的性能使设计的纳米和各向同性嵌段共聚物膜反应器在工业应用中极具吸引力。
