Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Sci Rep. 2024 Nov 25;14(1):29242. doi: 10.1038/s41598-024-78922-1.
Nanotechnology offers a novel strategy for enhancing the susceptibility of pathogens resistant to traditional antibiotics. Another effective strategy is combination therapy, where multiple agents are used together to improve treatment efficacy. In this study, both nanoparticle-based formulation and combinatorial therapy were utilized to develop a potent antibacterial system targeting infectious bacteria. Lysozyme (Lys) and Vancomycin (Van) were co-loaded onto mesoporous silica nanoparticles (MSNs), forming Lys-Van-MSNs. The antimicrobial activity of these nanoparticles was evaluated by determining the minimum inhibitory concentration (MIC) against Staphylococcus aureus. The MIC values for Lys-Van-MSNs were 0.85 µg/ml for Van and 0.168 mg/ml for Lys, reflecting reductions of 86.4% and 93.7%, respectively, compared to the free forms. Additionally, cytotoxicity was tested using MTT, ROS, and hemolysis assays on human cell lines (breast, fibroblast, and AGS), showing over 80% cell viability, indicating minimal toxicity. The MSN-based formulation, with its synergistic antibacterial effects, reduced drug dosage, and high biocompatibility, offers a practical and effective solution for addressing bacterial infections.
纳米技术为提高对抗生素具有耐药性的病原体的敏感性提供了一种新策略。另一种有效策略是联合治疗，即同时使用多种药物来提高治疗效果。在这项研究中，我们同时使用基于纳米颗粒的制剂和组合疗法，开发了一种针对感染性细菌的强效抗菌系统。溶菌酶（Lys）和万古霉素（Van）被共同负载到介孔硅纳米颗粒（MSNs）上，形成 Lys-Van-MSNs。通过测定最小抑菌浓度（MIC）来评估这些纳米颗粒的抗菌活性，以评估其对金黄色葡萄球菌的抑制作用。Lys-Van-MSNs 的 MIC 值为 0.85μg/ml（Van）和 0.168mg/ml（Lys），与游离形式相比，分别降低了 86.4%和 93.7%。此外，还通过 MTT、ROS 和溶血试验在人细胞系（乳腺、成纤维细胞和 AGS）上测试了细胞毒性，结果显示细胞活力超过 80%，表明毒性很小。基于 MSN 的制剂具有协同抗菌作用、减少药物剂量和高生物相容性，为解决细菌感染提供了一种实用且有效的解决方案。
