Bonney Julia R, Stratton Ariana E, Guo Yingchan, Eades Cabell B, Prentice Boone M
Department of Chemistry, University of Florida, Gainesville, Florida 32611, United States.
J Am Soc Mass Spectrom. 2025 Jan 1;36(1):119-126. doi: 10.1021/jasms.4c00368. Epub 2024 Nov 25.
Sulfatides are abundant components of the brain, and dysregulation of these molecules has been linked to several diseases. In sulfatide structures, a sugar is linked to a sphingoid backbone via an α-glycosidic or β-glycosidic linkage. While sulfatides are readily generated in negative ion mode imaging mass spectrometry experiments, resolving sulfatide diastereomers is challenging; therefore, identifications are usually reported as a single sulfatide. Herein, a gas-phase charge inversion ion/ion reaction between sulfatides and a strontium tris-phenanthroline [Sr(Phen)] reagent is performed to separate the diastereomers, as they form complexes containing different numbers of phenanthroline ligands. The ability to separate these diastereomers using the reaction alone, without the need for any further dissociation, allows for the workflow to be readily implemented in an imaging mass spectrometry experiment. Imaging mass spectrometry was performed on sulfatides generated directly from rat brain tissue, and both the α- and β-linked sulfatide images were obtained.
硫苷脂是大脑中丰富的成分，这些分子的失调与多种疾病有关。在硫苷脂结构中，一个糖通过α-糖苷键或β-糖苷键与一个鞘氨醇骨架相连。虽然硫苷脂在负离子模式成像质谱实验中很容易产生，但解析硫苷脂非对映异构体具有挑战性；因此，鉴定结果通常报告为单一硫苷脂。在此，进行了硫苷脂与三（菲咯啉）锶[Sr(Phen)]试剂之间的气相电荷反转离子/离子反应，以分离非对映异构体，因为它们形成了含有不同数量菲咯啉配体的配合物。仅使用该反应就能分离这些非对映异构体，而无需任何进一步的解离，这使得该工作流程能够很容易地在成像质谱实验中实施。对直接从大鼠脑组织产生的硫苷脂进行了成像质谱分析，并获得了α-和β-连接的硫苷脂图像。
