全基因组鉴定和表达分析揭示了 Platostoma palustre（Blume）A. J. Paton 中 WRKY 基因家族在非生物胁迫响应和多糖及类黄酮生物合成中的重要作用。

Genome-wide identification and expression analysis of the WRKY gene family reveal essential roles in abiotic stress responses and polysaccharides and flavonoids biosynthesis in Platostoma palustre (Blume) A. J. Paton.

机构信息

Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement/ Guangxi Engineering Research Center of TCM Resource Intelligent Creation, National Center for TCM Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, 530023, China.

School of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, 210000, China.

出版信息

BMC Plant Biol. 2024 Nov 26;24(1):1122. doi: 10.1186/s12870-024-05835-6.

DOI:10.1186/s12870-024-05835-6

PMID:39587501

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11590458/

Abstract

BACKGROUND

Platostoma palustre (Blume) A. J. Paton is an economic crop with medicinal and edible properties. WRKY transcription factors are widely involved in the growth and development, response to adversity stress, and hormone transduction of plants. The identification of the WRKY gene family has been completed in many plants, however, it has not yet been identified and analyzed at the genome-wide level in P. palustre.

RESULTS

In this study, we identified 133 PpWRKY gene family members (PpWRKYs) at the whole genome level of P. palustre, which were unevenly distributed on 15 chromosomes. Based on their protein structure and phylogenetic characteristics, the 133 PpWRKYs were divided into 3 subgroups. Segmental duplication events might play a crucial role in the expansion of the PpWRKY gene family. Through the transcriptome expression data analysis, the expression profiles of PpWRKY genes under Cd, red light, salt, and drought stresses were analyzed in this study, suggesting that WRKY transcription factors may play a crucial role in responding to different abiotic stresses in P. palustre. Notably, PpWRKY92 exhibited simultaneous responses to Cd, light intensity, salt, and drought stresses. Additionally, PpWRKY21, 75, 90, 52, 124, 39, 115, 122, 20, and 76 demonstrated a strong correlation with both monosaccharides and flavonoids. Taken together, PpWRKY20, 39, 75, 76, 90, 92, 115, 122, and 124 were found to be associated with the abiotic stress response and polysaccharides and flavonoids biosynthesis in P. palustre, except the low-expressed PpWRKY21 and 52.

CONCLUSION

The present study laid the foundation for the abiotic stress response and metabolite regulation of this gene family in P. palustre.

摘要

背景

Platostoma palustre (Blume) A. J. Paton 是一种具有药用和食用特性的经济作物。WRKY 转录因子广泛参与植物的生长发育、逆境胁迫响应和激素转导。许多植物的 WRKY 基因家族已经被鉴定出来，但在 Platostoma palustre 中尚未在全基因组水平上进行鉴定和分析。

结果

在本研究中，我们在 Platostoma palustre 的全基因组水平上鉴定了 133 个 PpWRKY 基因家族成员（PpWRKYs），它们不均匀地分布在 15 条染色体上。根据它们的蛋白质结构和系统发育特征，将 133 个 PpWRKY 分为 3 个亚组。片段复制事件可能在 PpWRKY 基因家族的扩张中发挥了关键作用。通过对转录组表达数据的分析，研究了 PpWRKY 基因在 Cd、红光、盐和干旱胁迫下的表达谱，表明 WRKY 转录因子可能在 Platostoma palustre 响应不同非生物胁迫中发挥关键作用。值得注意的是，PpWRKY92 对 Cd、光照强度、盐和干旱胁迫同时表现出响应。此外，PpWRKY21、75、90、52、124、39、115、122、20 和 76 与单糖和类黄酮表现出强烈的相关性。综合来看，PpWRKY20、39、75、76、90、92、115、122 和 124 与 Platostoma palustre 的非生物胁迫响应和多糖和类黄酮生物合成有关，除了低表达的 PpWRKY21 和 52。