Liu Chengming, Yang Yiting, Wang Meng, Jiang Wenyu, Du Yong, Hao Ziling, Chen Lei, Zhu Kangping, Liu Bin, Niu Lili, Zhao Ye, Wang Yan, Gan Mailin, Shen Linyuan, Zhu Li
State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Front Microbiol. 2024 Nov 11;15:1490064. doi: 10.3389/fmicb.2024.1490064. eCollection 2024.
-arginine is an α-amino acid and a semi-essential nutrient of significant biological interest. It plays a role in influencing various aspects of animal meat traits, gut microbiota composition, and physiological metabolism.
This study aimed to investigate the combined effects of -arginine supplementation on gut microbiota composition and the metabolism of the longissimus dorsi muscle in fattening pigs. Eighteen Yorkshire commercial pigs were divided into two groups: a control group that received no supplements and a treatment group that was given 1% -arginine for 52 days. The diversity and composition of microorganisms in the feces of the control (NC) and L-arginine (Arg) groups were analyzed by sequencing the 16S rRNA V3 -V4 region of the bacterial genome.
The findings indicated that -arginine supplementation increased both the abundance and diversity of gut microbiota, particularly affecting the Firmicutes and Bacteroidetes phyla. KEGG enrichment analysis revealed significant changes in several metabolism-related pathways, including amino acid, carbohydrate, and lipid metabolism. Metabolomic analysis identified 85 differential metabolites between the arginine and control groups, with phospholipids ranking among the top 20. Additionally, functional predictions indicated an increased abundance in the glycerophospholipid metabolism pathway. Correlation analysis linked changes in gut microbiota to phospholipid levels, which subsequently influenced post-slaughter meat color and drip loss.
These results suggest that -arginine supplementation positively impacts gut microbiota composition and the metabolic profile of the longissimus dorsi muscle in fattening pigs, with potential implications for meat quality.
L-精氨酸是一种α-氨基酸，是具有重要生物学意义的半必需营养素。它在影响动物肉质性状、肠道微生物群组成和生理代谢的各个方面发挥作用。
本研究旨在探讨补充L-精氨酸对育肥猪肠道微生物群组成和背最长肌代谢的联合影响。18头约克夏商品猪分为两组：不补充的对照组和给予1% L-精氨酸52天的处理组。通过对细菌基因组的16S rRNA V3-V4区域进行测序，分析对照组（NC）和L-精氨酸（Arg）组粪便中微生物的多样性和组成。
研究结果表明，补充L-精氨酸增加了肠道微生物群的丰度和多样性，尤其影响厚壁菌门和拟杆菌门。KEGG富集分析显示，包括氨基酸、碳水化合物和脂质代谢在内的几个与代谢相关的途径发生了显著变化。代谢组学分析确定了精氨酸组和对照组之间有85种差异代谢物，磷脂在前20种中。此外，功能预测表明甘油磷脂代谢途径的丰度增加。相关性分析将肠道微生物群的变化与磷脂水平联系起来，磷脂水平随后影响宰后肉色和滴水损失。
这些结果表明，补充L-精氨酸对育肥猪肠道微生物群组成和背最长肌的代谢谱有积极影响，对肉质有潜在影响。
