Lian Xian Qiang, Liu Guo Dong, Huang Miao Fen, Fan Qiu Hua, Lin Zi Dan
Dapeng New District Center for Disease Control and Prevention, Shenzhen, Guangdong, China.
Front Microbiol. 2024 Nov 11;15:1452942. doi: 10.3389/fmicb.2024.1452942. eCollection 2024.
() is a major food-borne pathogen which causes human gastroenteritis. Since the characteristics of remain unknown, 220 isolates selected from clinical and environmental samples in Dapeng of Shenzhen were tested for the presence of two hemolysin-expressing genes and . Among 27 clinical isolates, 26 carrired the gene, and the other one carried both and genes, however neither genes were detected in environmental isolates. Meanwhile, antimicrobial susceptibility profiles revealed the isolates with high frequency of resistance to ampicillin (77.73%) and colistin (71.82%) and medium to streptomycin (57.27%). Genetically, by whole genome sequencing (WGS), comparative genomics studies was performed on isolates from various districts and GenBank. Data analysis showed that antimicrobial resistance genes (ARGs) , and were harbored in all genomes and other ARGs was absent in the genomes of 27 clinical isolates. Besides, little regional difference was observed. As for virulence factors, MAM7, T3SS1, T3SS1 secret effector, T3SS2, T3SS2 secret effector, and VpadF were carried by most isolates. Two isolates from other districts were gene positive which clustered with clinical isolates from Dapeng in the same clade, indicating close genetic distance. This study revealed the widely distribution of in Shenzhen and the diverse ARGs and virulence genes it carried. Furthermore, pathways that pathogen disseminated through were discussed.
（）是一种主要的食源性病原体，可导致人类肠胃炎。由于（）的特征尚不清楚，对从深圳大鹏的临床和环境样本中选取的220株分离株进行了两种溶血素表达基因（）和（）的检测。在27株临床分离株中，26株携带（）基因，另一株同时携带（）和（）基因，但在环境分离株中均未检测到这两个基因。同时，抗菌药物敏感性分析显示，分离株对氨苄西林（77.73%）和黏菌素（71.82%）耐药频率较高，对链霉素耐药频率中等（57.27%）。在基因层面，通过全基因组测序（WGS），对来自不同地区的分离株和GenBank进行了比较基因组学研究。数据分析表明，所有基因组中均含有抗菌耐药基因（ARGs）（）、（）和（），而27株临床分离株的基因组中不存在其他ARGs。此外，未观察到明显的区域差异。至于毒力因子，大多数分离株携带MAM7、T3SS1、T3SS1分泌效应蛋白、T3SS2、T3SS2分泌效应蛋白和VpadF。来自其他地区的两株分离株（）基因呈阳性，与大鹏的临床分离株聚集在同一进化枝中，表明遗传距离较近。本研究揭示了（）在深圳的广泛分布及其携带的多种ARGs和毒力基因。此外，还讨论了病原体传播的途径。
