Nie Zhixiang, Cheng Xiangyu, Jiang Shengshi, Zhang Zhibin, Zhang Diwei, Chen Hanfang, Ling Na, Ye Yingwang
School of Food and Biological Engineering, Key Laboratory for Animal Food Green Manufacturing and Resource Mining of Anhui Province, Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei 230009, China.
Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
Foods. 2025 Jul 29;14(15):2660. doi: 10.3390/foods14152660.
() is a preeminent seafood-borne pathogen, imposing significant economic burdens on global aquaculture. The escalating prevalence of multidrug-resistant strains has accentuated the critical urgency for developing sustainable biocontrol strategies. In this study, a bacteriophage designated vB_VPAP_XY75 (XY75) was isolated and biologically characterized to establish an effective control against . . XY75 exhibited remarkable specificity toward , effectively lysing 46.2% of the target strains while showing no lytic activity against non-target bacterial species. Morphological characterization confirmed its taxonomic assignment to the family, featuring an icosahedral head (40 ± 2 nm) and contractile tail (60 ± 2 nm). XY75 demonstrated strong environmental tolerance, remaining stable at pH 4-11 and temperatures as high as 50 °C. At an optimal multiplicity of infection (MOI = 0.01), XY75 achieved a peak titer of 8.1 × 10 PFU/mL, a 5 min latent period, and burst size of 118 PFU/cell. Critically, XY75 reduced in salmon by more than 5.98 log CFU/g (99.9%) within 6 h at 4 °C, demonstrating exceptional cold tolerance and lytic activity. Genomic analysis confirmed that no virulence or antibiotic resistance genes were present. These results establish XY75 as a safe and efficacious biocontrol candidate for seafood preservation, with particular utility under refrigerated storage conditions.
（）是一种主要的食源性病原体，给全球水产养殖带来了巨大的经济负担。多重耐药菌株的日益流行凸显了制定可持续生物防治策略的紧迫性。在本研究中，分离出一种名为vB_VPAP_XY75（XY75）的噬菌体，并对其进行生物学特性鉴定，以建立针对……的有效控制方法。XY75对……表现出显著的特异性，能有效裂解46.2%的目标菌株，而对非目标细菌物种无裂解活性。形态学特征证实其归属于……科，具有二十面体头部（40±2nm）和收缩性尾部（60±2nm）。XY75表现出很强的环境耐受性，在pH值4 - 11和高达50°C的温度下仍保持稳定。在最佳感染复数（MOI = 0.01）下，XY75的滴度峰值达到8.1×10 PFU/mL，潜伏期为5分钟，裂解量为118 PFU/细胞。至关重要的是，在4°C下6小时内，XY75使鲑鱼体内的……减少了超过5.98 log CFU/g（99.9%），显示出卓越的耐寒性和裂解活性。基因组分析证实不存在毒力或抗生素抗性基因。这些结果表明XY75是一种安全有效的海产品保鲜生物防治候选物，在冷藏储存条件下具有特殊用途。
