NHC Key Laboratory of Food Safety Risk Assessment, China National Centre for Food Safety Risk Assessment, Beijing 100022, China.
College of Food Science and Engineering, Northwest A&F University, Shaanxi 712100, China.
Int J Food Microbiol. 2024 Jun 16;418:110737. doi: 10.1016/j.ijfoodmicro.2024.110737. Epub 2024 May 8.
Prevalent in marine, estuarine and coastal environments, Vibrio parahaemolyticus is one of the major foodborne pathogens which can cause acute gastroenteritis through consumption of contaminated food. This study encompassed antimicrobial resistance, molecular characteristics and phylogenetic relationships of 163 V. parahaemolyticus isolated from aquatic foods across 15 provinces in China. The isolates showed high resistance rates against ampicillin (90.80 %, 148/163) and cefazolin (72.39 %, 118/163). Only 5 isolates demonstrated multi-drug resistance (MDR) phenotypes. A total of 37 different antibiotic resistance genes (ARGs) in correlation with seven antimicrobial categories were identified. tet(34) and tet(35) were present in all 163 isolates. Other most prevalent ARGs were those conferring resistance to β-lactams, with prevalence rate around 18.40 % (30/163). The virulence genes tdh and trh were found in 17 (10.43 %) and 9 (5.52 %) isolates, respectively. Totally 121 sequence types (STs) were identified through whole genome analysis, among which 60 were novel. The most prevalent sequence type was ST3 (9.20 %, 15/163), which shared the same genotype profile of trh, tdh and bla. Most of the tdhV. parahaemolyticus isolates was clustered into a distinctive clade by the phylogenetic analysis. Our study showed that the antimicrobial resistance of V. parahaemolyticus in aquatic foods in China was moderate. However, the emerging of MDR isolates implicate strengthened monitoring is needed for the better treatment of human V. parahaemolyticus infections. High genetic diversity and virulence potential of the isolates analyzed in this study help better understanding and evaluating the risk of V. parahaemolyticus posed to public health.
副溶血性弧菌普遍存在于海洋、河口和沿海环境中，是主要的食源性致病菌之一，可通过食用被污染的食物引起急性肠胃炎。本研究涵盖了从中国 15 个省份的水产食品中分离出的 163 株副溶血性弧菌的抗菌药物耐药性、分子特征和系统发育关系。这些分离株对氨苄西林（90.80%，148/163）和头孢唑林（72.39%，118/163）的耐药率较高。只有 5 株分离株表现出多药耐药（MDR）表型。总共鉴定出与 7 种抗菌药物类别相关的 37 种不同的抗生素耐药基因（ARGs）。所有 163 株分离株均存在 tet(34)和 tet(35)。其他最常见的 ARGs 是那些赋予β-内酰胺类药物耐药性的基因，其流行率约为 18.40%（30/163）。毒力基因 tdh 和 trh 分别存在于 17（10.43%）和 9（5.52%）株分离株中。通过全基因组分析共鉴定出 121 种序列类型（STs），其中 60 种为新的。最常见的序列类型是 ST3（9.20%，15/163），其 trh、tdh 和 bla 的基因型相同。大多数 tdhV. parahaemolyticus 分离株通过系统发育分析聚类成一个独特的分支。本研究表明，中国水产食品中副溶血性弧菌的抗菌药物耐药性处于中等水平。然而，MDR 分离株的出现表明，需要加强监测，以更好地治疗人类副溶血性弧菌感染。本研究分析的分离株具有较高的遗传多样性和毒力潜力，有助于更好地了解和评估副溶血性弧菌对公众健康的风险。
