Zong Xinyu, Song Jiawen, Huang Xingting, Zhu Yuhang, Yu Haiwei, Ning Guogui, Zhao Jing
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan, China.
Pest Manag Sci. 2025 Mar;81(3):1579-1591. doi: 10.1002/ps.8561. Epub 2024 Nov 26.
The two-spotted spider mite, Tetranychus urticae, is a serious pest of glasshouse roses. The rapid development of resistance to acaricides has severely impacted rose production. To investigate the resistance status of T. urticae in glasshouse rose cultivation in China, we tested the susceptibility of 10 populations to 12 commonly used acaricides and examined the frequency of target-site mutations associated with acaricide resistance.
All of the tested populations showed resistance to at least eight types of acaricides and exhibited high levels of resistance to abamectin, diafenthiuron, hexythiazox and spirodiclofen. The JN-YN and LZ-GS populations even developed >70 000-fold resistance to abamectin. Additionally, resistance to the newly applied bifenazate or cyetpyrafen was detected in eight populations. Many glasshouse-collected populations showed significantly increased activity of detoxifying enzymes and harbored high frequencies of target-site mutations known to be associated with resistance. A newly discovered I136A mutation in cytb was detected at >85% frequencies in two populations with high levels of bifenazate resistance, and its frequency showed a strong correlation with the median lethal concentration values in all of the populations.
Multiple-acaricide resistance exists extensively in all collected populations. The high resistance levels of collected populations to many acaricides, together with multiple target-site mutations, indicate a serious resistance status of T. urticae in rose glasshouses in China. The role of the newly discovered I136A mutation in bifenazate resistance requires further verification. These results will be essential for implementing a well-thought-out resistance management program for rose cultivation in China. © 2024 Society of Chemical Industry.
二斑叶螨是温室玫瑰的一种严重害虫。对杀螨剂抗性的快速发展严重影响了玫瑰生产。为调查中国温室玫瑰种植中二斑叶螨的抗性状况，我们测试了10个种群对12种常用杀螨剂的敏感性，并检测了与杀螨剂抗性相关的靶标位点突变频率。
所有测试种群均对至少8种杀螨剂表现出抗性，且对阿维菌素、丁醚脲、噻螨酮和螺螨酯表现出高水平抗性。JN-YN和LZ-GS种群对阿维菌素甚至产生了>70000倍的抗性。此外，在8个种群中检测到对新使用的联苯肼酯或乙螨唑的抗性。许多温室采集的种群解毒酶活性显著增加，且携带已知与抗性相关的高频率靶标位点突变。在两个对联苯肼酯具有高水平抗性的种群中，细胞色素b中一个新发现的I136A突变的频率>85%，且其频率与所有种群的半数致死浓度值呈强相关。
所有采集种群中广泛存在多重杀螨剂抗性。采集种群对多种杀螨剂的高抗性水平以及多个靶标位点突变表明中国玫瑰温室中二斑叶螨的抗性状况严重。新发现的I136A突变在联苯肼酯抗性中的作用需要进一步验证。这些结果对于在中国实施周全的玫瑰种植抗性管理计划至关重要。© 2024化学工业协会。
