Liu Yaming, Liao Anjing, Chen Shunhong, Xu Ying, Zhou Jing-Jiang, Wu Jian
State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang 550025, China.
J Agric Food Chem. 2024 Dec 18;72(50):27619-27638. doi: 10.1021/acs.jafc.4c06407. Epub 2024 Nov 26.
Phytohormones, as crucial regulatory factors in plant growth and development, have garnered increased interest in improving crop stress resistance. It is essential to comprehend the distribution of phytohormones in plants to assess their health status and investigate their functions. This knowledge also serves as a guide for developing and using plant growth regulators. The advancement of fluorescent probe technology, along with the wide range of fluorophores and improvements in imaging methods, has made it a successful approach for monitoring phytohormones in plants. This technique has been confirmed to be effective in plants, particularly in detecting the response of fluorescent probes to phytohormones. In this Perspective, we highlight the utility of fluorescent probes in measuring and visualizing the distribution of phytohormones in plants under external stress. However, the visualization of phytohormones with high spatial resolution and the achievement of high biocompatibility in living plants have posed significant challenges for researchers. Nonetheless, there are also many untapped opportunities in this field. This paper seeks to delve into the potential for further discussion on the subject.
植物激素作为植物生长发育中的关键调控因子，在提高作物抗逆性方面越来越受到关注。了解植物激素在植物体内的分布对于评估植物健康状况和研究其功能至关重要。这些知识也为开发和使用植物生长调节剂提供了指导。荧光探针技术的进步，以及荧光团种类的丰富和成像方法的改进，使其成为监测植物体内植物激素的成功方法。该技术已在植物中得到证实是有效的，特别是在检测荧光探针对植物激素的响应方面。在这篇观点文章中，我们强调了荧光探针在测量和可视化外部胁迫下植物体内植物激素分布方面的实用性。然而，在活植物中实现高空间分辨率的植物激素可视化以及高生物相容性对研究人员构成了重大挑战。尽管如此，该领域也有许多尚未开发的机会。本文旨在深入探讨该主题进一步讨论的潜力。
