Koshakji R P, Wood A J
J Pharm Sci. 1986 Jan;75(1):87-9. doi: 10.1002/jps.2600750121.
A simple, sensitive, and rapid fluorometric-high-performance liquid chromatographic method for quantitating human and animal plasma and blood levels of propranolol has been developed. The procedure, which requires microliter volumes of triethylamine in the mobile phase mixture and microliter volumes of plasma or blood (50-100 microL), may be applied in routine clinical analysis as well as in pharmacokinetic studies using small laboratory animals. A single, selective extraction step precedes the analysis. The limiting concentration that can be detected is 1 ng/mL.
已开发出一种简单、灵敏且快速的荧光高效液相色谱法，用于定量测定人及动物血浆和血液中普萘洛尔的含量。该方法在流动相混合物中只需微升体积的三乙胺，且血浆或血液只需微升体积（50 - 100微升），可应用于常规临床分析以及使用小型实验动物的药代动力学研究。分析前只需进行一步选择性萃取。可检测的极限浓度为1纳克/毫升。
