Nygard G, Shelver W H, Wahba Khalil S K
J Pharm Sci. 1979 Mar;68(3):379-81. doi: 10.1002/jps.2600680336.
A high-pressure liquid chromatographic method is presented for the determination of propranolol in human plasma. A reversed-phase cyanopropylsilane column was utilized with a liquid phase consisting of 70% acetonitrile and 30% 0.02 M acetate buffer, pH 7.0. A spectrofluorometric detector with an excitation wavelength of 276 nm and an emission filter with a 340-nm cutoff provided a detectable peak for 0.8 ng of propranolol/injection with this system. The reproducibility and precision of the method are shown from the analyses of samples containing 10--150 ng/ml of plasma.
本文介绍了一种用于测定人血浆中普萘洛尔的高压液相色谱法。采用反相氰丙基硅烷柱，流动相由70%乙腈和30% 0.02M醋酸盐缓冲液（pH 7.0）组成。该系统使用激发波长为276nm的荧光分光光度计检测器和截止波长为340nm的发射滤光片，每次进样0.8ng普萘洛尔时可检测到峰。通过对含10 - 150ng/ml血浆的样品进行分析，展示了该方法的重现性和精密度。
