Fakhari Sepideh, Belleannée Clémence, Charrette Steve J, Greener Jesse
Department of Chemistry, Faculty of Science and Engineering, Université Laval, Québec City, QC G1V 0A6, Canada.
Centre de Recherche du Centre Hospitalier Universitaire de Québec, Université Laval, Québec City, QC G1V 0A6, Canada.
Biomimetics (Basel). 2024 Oct 27;9(11):657. doi: 10.3390/biomimetics9110657.
Shear stress plays a crucial role in modulating cell adhesion and signaling. We present a microfluidic shear stress generator used to investigate the adhesion dynamics of , an amoeba cell model organism with well-characterized adhesion properties. We applied shear stress and tracked cell adhesion, motility, and detachment using time-lapse videomicroscopy. In the precise shear conditions generated on-chip, our results show cell migration patterns are influenced by shear stress, with cells displaying an adaptive response to shear forces as they alter their adhesion and motility behavior. Additionally, we observed that DH1-10 wild-type cells exhibit stronger adhesion and resistance to shear-induced detachment compared to adhesion-defective mutant cells. We also highlight the influence of cell density on detachment kinetics.
剪切应力在调节细胞黏附和信号传导中起着关键作用。我们展示了一种微流控剪切应力发生器，用于研究一种具有明确黏附特性的变形虫细胞模型生物的黏附动力学。我们施加剪切应力，并使用延时视频显微镜跟踪细胞的黏附、运动和脱离。在芯片上产生的精确剪切条件下，我们的结果表明细胞迁移模式受剪切应力影响，细胞在改变其黏附和运动行为时对剪切力表现出适应性反应。此外，我们观察到与黏附缺陷突变细胞相比，DH1-10野生型细胞表现出更强的黏附力和对剪切诱导脱离的抵抗力。我们还强调了细胞密度对脱离动力学的影响。
