Paladini Federica, Russo Francesca, Masi Annalia, Lanzillotti Carmen, Sannino Alessandro, Pollini Mauro
Department of Experimental Medicine, University of Salento, Via Monteroni, 73100 Lecce, Italy.
Caresilk S.r.l.s., c/o Dhitech, Via Monteroni, 73100 Lecce, Italy.
Biomimetics (Basel). 2024 Oct 29;9(11):659. doi: 10.3390/biomimetics9110659.
The risk of infections in chronic wounds represents a serious issue, particularly in aged people and in patients affected by diseases such as diabetes and obesity. Moreover, the growing resistance demonstrated by many bacterial strains has significantly reduced the therapeutic options for clinicians and has become a great challenge for the researchers in the definition of novel approaches that promote the wound healing process and reduce the healing time. Tissue engineering approaches based on biomaterials and three-dimensional scaffolds have demonstrated huge potential in supporting cell proliferation; among them, Bombyx mori-derived silk fibroin is a very appealing possibility for the development of devices with regenerative properties for wound healing applications. However, due to the high risk of infections in chronic wounds, an antibacterial treatment is also strongly encouraged for preventing bacterial proliferation at the wound site. In this work, to develop a device with regenerative and antibacterial properties, antibacterial silver coatings were deposited onto silk fibroin scaffolds, and the effect of the treatment in terms of chemical-physical and microbiological properties was investigated. The results demonstrated that the silver treatment improved the mechanical properties of the protein scaffold and provided good antibacterial efficacy against representative bacterial strains in wound infection, namely and antibiotic-resistant .
慢性伤口感染的风险是一个严重问题，尤其在老年人以及患有糖尿病和肥胖症等疾病的患者中。此外，许多细菌菌株表现出的日益增强的耐药性显著减少了临床医生的治疗选择，并且对于研究人员而言，在定义促进伤口愈合过程并缩短愈合时间的新方法方面已成为一项巨大挑战。基于生物材料和三维支架的组织工程方法在支持细胞增殖方面已显示出巨大潜力；其中，家蚕衍生的丝素蛋白对于开发具有再生特性的伤口愈合应用设备而言是一种非常有吸引力的选择。然而，由于慢性伤口感染风险高，强烈鼓励进行抗菌治疗以防止伤口部位细菌增殖。在这项工作中，为开发一种具有再生和抗菌特性的设备，将抗菌银涂层沉积在丝素蛋白支架上，并研究了该处理在化学物理和微生物学特性方面的效果。结果表明，银处理改善了蛋白质支架的机械性能，并对伤口感染中的代表性细菌菌株，即 和耐抗生素菌株，提供了良好的抗菌效果。
