Shi Ruiguo, Jin Guimei, Shen Shicai, Xu Gaofeng, Zheng Fengping, Clements David Roy, Yang Yunhai, Yang Shaosong, Wan Fanghao, Zhang Fudou, Liu Bo
College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China.
Key Laboratory of Prevention and Control of Biological Invasions, Ministry of Agriculture and Rural Affairs of China, Agricultural Environment and Resource Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China.
Curr Issues Mol Biol. 2024 Oct 23;46(11):11890-11905. doi: 10.3390/cimb46110706.
Sweet potato ( L.) is one of the most important global food crops. This crop exhibits excellent allelopathic potential against various weeds, but its allelopathic mechanism at the molecular level is unclear. Therefore, metabolomic and transcriptomic analyses were performed to explore the allelopathic effects, metabolic pathway, and associated genes for two major compounds with allelopathic activity, palmitic acid and linoleic acid. The sweet potato variety Ningshu 25 was employed in the current study. The results showed that palmitic acid and linoleic acid had strong allelopathic effects on seed germination, plant growth, antioxidant enzyme activity, and chlorophyll content of two weeds and . The content of the two targeted metabolites was affected by different environmental conditions and was significantly increased under low temperature (15 °C). Five metabolic pathways involved in the two targeted metabolites of fatty acids were found: fatty acid biosynthesis, fatty acid elongation, fatty acid degradation, biosynthesis of cutin, suberine, and wax, and the linoleic acid metabolism pathway. The synthesis of palmitic acid is significantly enriched in the biosynthesis pathways of fatty acids, cutin, suberine, and wax, and the synthesis of linoleic acid is significantly enriched in the linoleic acid metabolism pathway. Under different environmental conditions, there were three key genes expressed-g4988, g11881, and g19673-located in the biosynthesis pathways of cutin, suberine, and wax; four key genes expressed-g31191, g60956, g49811, and g59542-located in the biosynthesis pathway of fatty acids; and six key expressed genes-g26575, g24787, g23517, g57649, g58562, and g4314-located in biosynthesis pathway of linoleic acid, respectively. Our study advances understanding of the molecular mechanisms behind allelopathic traits in sweet potato and provides a set of candidate genes for use in improving allelopathic potential in sweet potato germplasm resources.
甘薯（Ipomoea batatas (L.)）是全球最重要的粮食作物之一。这种作物对多种杂草具有优异的化感潜力，但其分子水平的化感机制尚不清楚。因此，进行了代谢组学和转录组学分析，以探究两种具有化感活性的主要化合物——棕榈酸和亚油酸的化感效应、代谢途径及相关基因。本研究采用甘薯品种宁薯25。结果表明，棕榈酸和亚油酸对两种杂草的种子萌发、植株生长、抗氧化酶活性及叶绿素含量具有强烈的化感效应。这两种目标代谢物的含量受不同环境条件影响，在低温（15℃）下显著增加。发现了与脂肪酸的两种目标代谢物相关的五条代谢途径：脂肪酸生物合成、脂肪酸延长、脂肪酸降解、角质、木栓质和蜡的生物合成以及亚油酸代谢途径。棕榈酸的合成在脂肪酸、角质、木栓质和蜡的生物合成途径中显著富集，亚油酸的合成在亚油酸代谢途径中显著富集。在不同环境条件下，有三个关键基因g4988、g11881和g19673在角质、木栓质和蜡的生物合成途径中表达；四个关键基因g31191、g60956、g49811和g59542在脂肪酸生物合成途径中表达；六个关键基因g26575、g24787、g23517、g57649、g58562和g4314分别在亚油酸生物合成途径中表达。我们的研究推进了对甘薯化感特性背后分子机制的理解，并为提高甘薯种质资源的化感潜力提供了一组候选基因。
