Du Kebing, Xu Youming, Wang Ningning, Qin Liyuan, Tao Jieyun
College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China.
Curr Issues Mol Biol. 2024 Oct 23;46(11):11927-11941. doi: 10.3390/cimb46110708.
has been extensively cultivated in the wetlands of the Yangtze River in south China and has significantly contributed to ecology and timber production. Until now, research on genomics has yet to be conducted due to its large and complex genome, which hinders the development of genomic resources. Combined with the microstructural changes during cambium cell differentiation across various growth periods, we investigate the transcriptome expression and regulatory mechanisms governing cambium activity in . Using RNA sequencing (RNA-Seq) technology, we identified the genes involved in the cambium development of cells at three stages (dormancy, reactivation, and activity). These genes encode the regulatory and control factors associated with the cambial activity, cell division, cell expansion, and biosynthesis of cell wall components. Blast comparison revealed that three genes (, , ) from the and families might regulate transcription during lignin formation in wood thickening. These results illustrate the dynamic changes in the transcriptional network during vascular cambium development. Additionally, they shed light on the genetic regulation mechanism of secondary growth in and guide further elucidation of the candidate genes involved in regulating cambium differentiation and wood formation.
在中国南方长江流域的湿地中广泛种植，对生态和木材生产做出了重大贡献。到目前为止，由于其基因组庞大且复杂，阻碍了基因组资源的开发，尚未对其进行基因组学研究。结合不同生长时期形成层细胞分化过程中的微观结构变化，我们研究了形成层活性的转录组表达和调控机制。利用RNA测序（RNA-Seq）技术，我们鉴定了细胞形成层发育三个阶段（休眠、重新激活和活动）中涉及的基因。这些基因编码与形成层活性、细胞分裂、细胞扩张和细胞壁成分生物合成相关的调控因子。Blast比较显示，来自[具体家族名称]和[具体家族名称]家族的三个基因（[基因名称1]、[基因名称2]、[基因名称3]）可能在木材增厚过程中木质素形成期间调节转录。这些结果说明了维管形成层发育过程中转录网络的动态变化。此外，它们揭示了[植物名称]次生生长的遗传调控机制，并指导进一步阐明参与调节形成层分化和木材形成的候选基因。
