Estep Alden S, Sanscrainte Neil D, Lamberg Frieda, McStoots Darrel, Gosselin Susan
Mosquito & Fly Research Unit, Center for Medical, Agricultural and Veterinary Entomology, United States Department of Agriculture, 1700 SW 23rd Drive, Gainesville, FL 32608, USA.
Entomology and Pest Control Section, Bureau of Scientific Evaluation and Technical Assistance, Division of Agricultural Environmental Services, Florida Department of Agriculture and Consumer Services, 3125 Conner Boulevard, Tallahassee, FL 32399, USA.
Insects. 2024 Nov 4;15(11):863. doi: 10.3390/insects15110863.
is a major arboviral disease vector and is therefore a critical target for control by public health programs. Early eradication efforts have shown that can rapidly build insecticide resistance, and, now, resistance to pyrethroids, the major class of adulticides used for operational control, is common in many populations. A major contributor to this resistance is a group of knockdown resistance () SNPs that normally exist in distinct ensembles in the Western Hemisphere and the Indopacific with little known mixing. In this study, we detected, and confirmed, using multiple methods, the Indopacific SNPs, both V1016G and S989P, in three recent collections from Osceola County, Florida. This represents a large expansion of the geographic range of the Indopacific SNPs. We consider the implications of this finding on future insecticide resistance surveillance studies, including assessing the ability of our current screening tools to detect these SNPs. We find that the presence of the Indopacific SNPs requires the modification of existing resistance screening protocols and requires further work to understand the operational implications for mosquito control.
是一种主要的虫媒病毒病媒介，因此是公共卫生项目控制的关键目标。早期的根除努力表明，其能够迅速产生杀虫剂抗性，如今，对用于实际控制的主要一类成人杀虫剂拟除虫菊酯的抗性在许多种群中很常见。这种抗性的一个主要促成因素是一组击倒抗性（）单核苷酸多态性，它们通常在西半球和印度太平洋地区以不同的组合形式存在，很少有混合的情况。在本研究中，我们使用多种方法在佛罗里达州奥西奥拉县最近的三个样本中检测并确认了印度太平洋地区的单核苷酸多态性，即V1016G和S989P。这代表着印度太平洋地区单核苷酸多态性地理范围的大幅扩展。我们考虑了这一发现对未来杀虫剂抗性监测研究的影响，包括评估我们当前的筛查工具检测这些单核苷酸多态性的能力。我们发现，印度太平洋地区单核苷酸多态性的存在需要修改现有的抗性筛查方案，并需要进一步开展工作以了解对蚊虫控制的实际影响。
