Cui Jinhao, Makita Yoshimasa, Okamura Tomoharu, Ikeda Chihoko, Fujiwara Shin-Ichi, Tominaga Kazuya
Department of Oral Pathology, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata 573-1121, Osaka, Japan.
Department of Chemistry, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata 573-1121, Osaka, Japan.
J Funct Biomater. 2024 Nov 7;15(11):333. doi: 10.3390/jfb15110333.
Oral squamous cell carcinoma (OSCC) is a common malignancy in the oral cavity. Photodynamic therapy (PDT) is a new alternative for the treatment of diseases using photosensitizers (PS) and light. In this study, we used a photosensitizer complex (Ce6-MnNPs-Chlorin e6 combined with up-conversion nanoparticles NaYF:Yb/Er/Mn) to investigate the therapeutic effectiveness of this treatment against oral cancer cells. We also investigated the mechanism of action of near-infrared light PDT (NIR-PDT) combined with the Ce6-MnNPs. After determining a suitable concentration of Ce6-MnNPs using an MTT assay, human oral squamous cell carcinoma cells (HSC-3) were treated with NIR-PDT with Ce6-MnNPs. We examined the characteristics of Ce6-MnNPs by transmission electron microscopy (TEM); a zeta potential and particle size analyzer; Fourier-transform infrared spectroscopy (FTIR); cell viability by MTT assay; and apoptosis by FITC-Annexin V/PI assay. The mitochondrial membrane potential (MMP), apoptosis-related mRNA level (Bax and Bcl-2) and p53 protein were also researched. NIR-PDT with 0.5 ng/µL Ce6-MnNPs inhibited the proliferation of HSC-3 ( < 0.05). After treatment with NIR-PDT, changes in the mitochondrial membrane potential and apoptosis occurred ( < 0.01). The ratio of Bax/Bcl-2 and p53-positive cells increased ( < 0.01). These results suggest that this treatment can induce apoptosis of oral cancer cells.
口腔鳞状细胞癌（OSCC）是口腔中常见的恶性肿瘤。光动力疗法（PDT）是一种使用光敏剂（PS）和光治疗疾病的新方法。在本研究中，我们使用了一种光敏剂复合物（Ce6-MnNPs-叶绿素e6与上转换纳米颗粒NaYF:Yb/Er/Mn结合）来研究这种治疗方法对口腔癌细胞的治疗效果。我们还研究了近红外光动力疗法（NIR-PDT）与Ce6-MnNPs联合作用的机制。使用MTT法确定合适的Ce6-MnNPs浓度后，用含Ce6-MnNPs的NIR-PDT处理人口腔鳞状细胞癌细胞（HSC-3）。我们通过透射电子显微镜（TEM）、zeta电位和粒径分析仪、傅里叶变换红外光谱（FTIR）来检测Ce6-MnNPs的特性；通过MTT法检测细胞活力；通过FITC-Annexin V/PI法检测细胞凋亡。还研究了线粒体膜电位（MMP）、凋亡相关mRNA水平（Bax和Bcl-2）和p53蛋白。含0.5 ng/µL Ce6-MnNPs的NIR-PDT抑制了HSC-3的增殖（P<0.05）。用NIR-PDT处理后，线粒体膜电位发生变化并出现凋亡（P<0.01）。Bax/Bcl-2比值和p53阳性细胞增加（P<0.01）。这些结果表明这种治疗可诱导口腔癌细胞凋亡。
