MOE Key Laboratory of Evolution & Marine Biodiversity, Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
MOE Key Laboratory of Marine Genetics & Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
Mar Drugs. 2024 Oct 31;22(11):492. doi: 10.3390/md22110492.
Members of the genus are widely distributed in the marine environment and are known for their ability to produce tropodithietic acid (TDA). Studies investigating the genomic and metabolic features of strains from marine biofilms are sparse. Here, we analyze the complete genomes of 18 strains isolated from biofilms on subtidal stones, with the aim of determining their potential to synthesize secondary metabolites. Based on whole-genome comparison and average nucleotide identity calculation, the isolated bacteria are classified as novel strains of . Further analysis reveals a total of 153 biosynthetic gene clusters, which are assigned to 32 gene cluster families with low similarity to previously published ones. Complete TDA clusters are identified in 14 of the 18 strains, while in the other 4 strains the TDA clusters are rather incomplete and scattered across different chromosome and plasmid locations. Phylogenetic analysis suggests that their presence or absence may be potentially attributed to horizontal gene transfer. High-performance liquid chromatography-mass spectrometry analysis demonstrates the production of TDA in all the examined strains. Furthermore, the strains have strong antibacterial activity against the pathogenic strain ems001, which is associated with acute hepatopancreatic necrosis in South American white shrimp. Altogether, this study ameliorates our knowledge of marine biofilm-associated and offers new avenues for exploiting marine antimicrobial agents.
属的成员广泛分布于海洋环境中，以能够产生 tropodithietic 酸 (TDA) 而闻名。研究海洋生物膜中 菌株的基因组和代谢特征的研究很少。在这里，我们分析了从潮间带石块生物膜中分离的 18 株完整基因组，旨在确定它们合成次生代谢物的潜力。基于全基因组比较和平均核苷酸同一性计算，分离出的细菌被分类为 的新型菌株。进一步分析显示，总共鉴定出 153 个生物合成基因簇，这些基因簇分为 32 个基因簇家族，与之前发表的基因簇家族相似度较低。在 18 株中的 14 株中鉴定出完整的 TDA 簇，而在其他 4 株中，TDA 簇相当不完整，分布在不同的染色体和质粒位置。系统发育分析表明，它们的存在或缺失可能归因于水平基因转移。高效液相色谱-质谱分析表明，所有被检测的菌株都能产生 TDA。此外，这些 菌株对与南美白对虾急性肝胰腺坏死相关的致病菌 ems001 具有很强的抗菌活性。总的来说，这项研究增进了我们对海洋生物膜相关 的认识，并为开发海洋抗菌剂提供了新的途径。
