School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, China.
College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China.
Mar Drugs. 2024 Nov 20;22(11):521. doi: 10.3390/md22110521.
Chemically investigating the marine-derived 1268 led to the isolation of a new compound of carpatamide I (). Subsequent genomic analysis identified its candidate biosynthetic gene cluster of approximately 44 kb. In order to obtain more carpatamide derivatives, we conducted the upregulation of Ctd14, which is a positive regulator, and obtained improvement of carpatamide I and four new compounds of carpatamides J-M (-). The structures of the aforementioned five new isolates were identified by a combination of ESI-HRMS as well as one-dimensional (1D) and two-dimensional (2D) spectral NMR datasets. Bioassay results showed that compounds - displayed anti-inflammatory activity and weak cytotoxicity against cell lines of A549, HT-29, and HepG2.
对海洋来源的 1268 进行化学研究，导致分离出一种新型化合物 carpatamide I ()。随后的基因组分析确定了其候选生物合成基因簇，约为 44 kb。为了获得更多的 carpatamide 衍生物，我们上调了正调控因子 Ctd14，得到了 carpatamide I 的改善和四种新型化合物 carpatamides J-M ()。通过 ESI-HRMS 以及一维 (1D) 和二维 (2D) 光谱 NMR 数据集的组合，确定了上述五个新分离物的结构。生物测定结果表明，化合物 - 具有抗炎活性和对 A549、HT-29 和 HepG2 细胞系的弱细胞毒性。
