CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Appl Microbiol Biotechnol. 2020 Jan;104(1):67-76. doi: 10.1007/s00253-019-10227-0. Epub 2019 Nov 26.
Marine Streptomyces sp. are an important source of bioactive compounds owing to their unique habitats and metabolic pathways. Whole-genome sequencing and bioinformatics analyses have shown that the potential of synthesizing secondary metabolites from marine-derived Streptomyces has been substantially underestimated. Genome mining is an integrated strategy used to discover natural products based on gene cluster sequences and biosynthetic pathways. Its emergence has greatly enhanced the discovery of natural compounds from marine Streptomyces, thereby yielding a large number of bioactive molecules with novel structures and potent activities. In this review, we briefly summarize the current applications of genome mining in marine Streptomyces, such as bioinformatics-based optimization of culture conditions, ribosome engineering, control of regulatory networks, heterologous expression of biosynthetic gene cluster, and combinatorial biosynthesis of natural compounds. Furthermore, we discuss the factors hindering the utilization of marine-derived natural products and conclude with the prospects for this technique.
海洋链霉菌由于其独特的生境和代谢途径，是生物活性化合物的重要来源。全基因组测序和生物信息学分析表明，海洋来源的链霉菌合成次生代谢产物的潜力被大大低估了。基因组挖掘是一种基于基因簇序列和生物合成途径发现天然产物的综合策略。它的出现极大地促进了海洋链霉菌中天然化合物的发现，从而产生了大量具有新颖结构和强大活性的生物活性分子。在本文中，我们简要总结了基因组挖掘在海洋链霉菌中的应用，如基于生物信息学的培养条件优化、核糖体工程、调控网络控制、生物合成基因簇的异源表达和天然化合物的组合生物合成。此外，我们还讨论了阻碍海洋天然产物利用的因素，并对该技术的前景进行了总结。
