（Pol d 2）、（Ves v 2）和 Venom（Api m 2）中的透明质酸酶的变应原性比较评估。

Comparative Assessment of the Allergenicity of Hyaluronidases from (Pol d 2), (Ves v 2), and Venom (Api m 2).

机构信息

Center of Allergy and Environment (ZAUM), Technical University of Munich, School of Medicine and Health & Helmholtz Munich, German Research Center for Environmental Health, 85764 Munich, Germany.

Department of Dermatology and Allergy Biederstein, School of Medicine and Health, Technical University of Munich, 80802 Munich, Germany.

出版信息

Toxins (Basel). 2024 Nov 19;16(11):498. doi: 10.3390/toxins16110498.

DOI:10.3390/toxins16110498

PMID:39591253

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11598713/

Abstract

Sensitization to cross-reactive allergens complicates identifying the culprit insect in Hymenoptera venom allergy via diagnostic tests. This study evaluates sensitization to hyaluronidases (Api m 2 from honey bee () venom, HBV; Pol d 2 from European paper wasp () venom, PDV; and Ves v 2.0101 and Ves v 2.0201 from yellow jacket () venom, YJV) and their cross-reactivity in allergic patients from Italy, Spain, and Germany using ImmunoCAPs, ELISA, and basophil activation tests. Sensitization rates were 45% for Api m 2 in HBV-allergic subjects, 25% for Pol d 2 in PDV-allergic individuals, and 20% and 10% for Ves v 2.0201 and Ves v 2.0101 in YJV-allergic patients, respectively. Patients primarily sensitized to Api m 2 showed minimal cross-reactivity to vespid hyaluronidases, whereas those primarily sensitized to Pol d 2 or Ves v 2.0201 exhibited IgE reactivity to Api m 2. Neither Pol d 2 nor Ves v 2.0201 triggered basophil activation. Cross-reactivity of Api m 2, Pol d 2, and Ves v 2.0201 depends on the primary sensitizing venom. Sensitization to Pol d 2 and Ves v 2.0201 remains below 25%, yet these patients may exhibit cross-reactivity to Api m 2. Conversely, HBV-allergic patients sensitized to Api m 2 show minimal reactivity to Pol d 2 or Ves v 2.0201.

摘要

对交叉反应性过敏原的致敏作用使通过诊断测试鉴定膜翅目毒液过敏中的罪魁祸首昆虫变得复杂。本研究使用 ImmunoCAPs、ELISA 和嗜碱性粒细胞活化试验评估了来自意大利、西班牙和德国的过敏患者对透明质酸酶（来自蜜蜂毒液的 Api m 2 ()、HBV；来自欧洲胡蜂毒液的 Pol d 2 ()、PDV；以及来自黄蜂毒液的 Ves v 2.0101 和 Ves v 2.0201，YJV）的致敏情况及其交叉反应性。HBV 过敏患者中 Api m 2 的致敏率为 45%，PDV 过敏个体中 Pol d 2 的致敏率为 25%，YJV 过敏患者中 Ves v 2.0201 和 Ves v 2.0101 的致敏率分别为 20%和 10%。主要对 Api m 2 致敏的患者对黄蜂透明质酸酶的交叉反应性最小，而主要对 Pol d 2 或 Ves v 2.0201 致敏的患者则对 Api m 2 表现出 IgE 反应性。Pol d 2 和 Ves v 2.0201 均未引发嗜碱性粒细胞活化。Api m 2、Pol d 2 和 Ves v 2.0201 的交叉反应性取决于主要致敏毒液。Pol d 2 和 Ves v 2.0201 的致敏率仍低于 25%，但这些患者可能表现出对 Api m 2 的交叉反应性。相反，对 Api m 2 致敏的 HBV 过敏患者对 Pol d 2 或 Ves v 2.0201 的反应性最小。