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高分子量二肽基肽酶 IV Pol d 3 是 Polistes dominula 毒液的主要过敏原。

The high molecular weight dipeptidyl peptidase IV Pol d 3 is a major allergen of Polistes dominula venom.

机构信息

Center of Allergy and Environment (ZAUM), Technical University of Munich and Helmholtz Center Munich, Member of the German Center of Lung Research (DZL), Munich, Germany.

Department of Infection and Immunity, Luxembourg Institute of Health (LIH), Esch-sur-Alzette, Luxembourg.

出版信息

Sci Rep. 2018 Jan 22;8(1):1318. doi: 10.1038/s41598-018-19666-7.

DOI:10.1038/s41598-018-19666-7
PMID:29358620
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5778000/
Abstract

Hymenoptera venom allergy can cause severe anaphylaxis in untreated patients. Polistes dominula is an important elicitor of venom allergy in Southern Europe as well as in the United States. Due to its increased spreading to more moderate climate zones, Polistes venom allergy is likely to gain importance also in these areas. So far, only few allergens of Polistes dominula venom were identified as basis for component-resolved diagnostics. Therefore, this study aimed to broaden the available panel of important Polistes venom allergens. The 100 kDa allergen Pol d 3 was identified by mass spectrometry and found to be a dipeptidyl peptidase IV. Recombinantly produced Pol d 3 exhibited sIgE-reactivity with approximately 66% of Polistes venom-sensitized patients. Moreover, its clinical relevance was supported by the potent activation of basophils from allergic patients. Cross-reactivity with the dipeptidyl peptidases IV from honeybee and yellow jacket venom suggests the presence of exclusive as well as conserved IgE epitopes. The obtained data suggest a pivotal role of Pol d 3 as sensitizing component of Polistes venom, thus supporting its status as a major allergen of clinical relevance. Therefore, Pol d 3 might become a key element for proper diagnosis of Polistes venom allergy.

摘要

膜翅目毒液过敏在未经治疗的患者中可引起严重的过敏反应。Polistes dominula 是欧洲南部和美国毒液过敏的重要过敏原。由于其传播到更温和气候带的增加，Polistes 毒液过敏也可能在这些地区变得更为重要。到目前为止，仅鉴定出少数 Polistes dominula 毒液过敏原作为组分分辨诊断的基础。因此，本研究旨在扩大重要的 Polistes 毒液过敏原的现有面板。通过质谱鉴定出 100kDa 过敏原 Pol d 3，发现其为二肽基肽酶 IV。重组产生的 Pol d 3 与大约 66%的 Polistes 毒液致敏患者的 sIgE 反应。此外，来自过敏患者的嗜碱性粒细胞的强烈激活支持其临床相关性。与蜜蜂和黄蜂毒液中的二肽基肽酶 IV 的交叉反应表明存在独特和保守的 IgE 表位。获得的数据表明 Pol d 3 作为 Polistes 毒液的致敏成分具有关键作用，因此支持其作为具有临床相关性的主要过敏原的地位。因此，Pol d 3 可能成为正确诊断 Polistes 毒液过敏的关键要素。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04f3/5778000/cd4c24463421/41598_2018_19666_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04f3/5778000/95a3a74bc5aa/41598_2018_19666_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04f3/5778000/5b52f3543f2b/41598_2018_19666_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04f3/5778000/f1a1eaebf79b/41598_2018_19666_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04f3/5778000/cf78c57663e6/41598_2018_19666_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04f3/5778000/cd4c24463421/41598_2018_19666_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04f3/5778000/95a3a74bc5aa/41598_2018_19666_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04f3/5778000/5b52f3543f2b/41598_2018_19666_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04f3/5778000/f1a1eaebf79b/41598_2018_19666_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04f3/5778000/cf78c57663e6/41598_2018_19666_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04f3/5778000/cd4c24463421/41598_2018_19666_Fig5_HTML.jpg

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