Xiao Xiuqing, Xu Fuqiang, Jia Fan
Shenzhen Key Laboratory of Viral Vectors for Biomedicine, Shenzhen-Hong Kong Institute of Brain Science, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Key Laboratory of Quality Control Technology for Virus-Based Therapeutics, The Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Vet Sci. 2024 Oct 30;11(11):529. doi: 10.3390/vetsci11110529.
FHV-1 is a highly contagious pathogen that significantly threatens feline health and contributes to rising pet healthcare costs. The mechanisms underlying FHV-1 and host interactions remain poorly understood. For the first time, we conducted a systematic analysis of transcriptomic changes in CRFK cells following FHV-1 infection using RNA-seq. The differentially expressed genes (DEGs) displayed significant associations with cellular components, particularly the chromatin structure. Pathway analysis of the DEGs highlighted key host immune responses, including Toll-like receptors (TLRs), IL-17, TNF, MAPK, and Rap1 signaling pathways. By integrating the RNA-seq and RT-qPCR results, we identified CXCL8, CXCL10, MMP1, MMP9, CSF2, CSF3, CCL20, TLR2, TLR3, TLR4, TNF, and FOS as potentially important genes in the host's immune response to FHV-1. These findings provide valuable insights into the mechanisms underlying FHV-1 and host interactions.
猫疱疹病毒1型（FHV-1）是一种极具传染性的病原体，严重威胁猫的健康，并导致宠物医疗成本不断上升。FHV-1与宿主相互作用的潜在机制仍知之甚少。我们首次使用RNA测序对FHV-1感染后的CRFK细胞转录组变化进行了系统分析。差异表达基因（DEGs）与细胞成分，特别是染色质结构显示出显著关联。对DEGs的通路分析突出了关键的宿主免疫反应，包括Toll样受体（TLRs）、IL-17、TNF、MAPK和Rap1信号通路。通过整合RNA测序和RT-qPCR结果，我们确定CXCL8、CXCL10、MMP1、MMP9、CSF2、CSF3、CCL20、TLR2、TLR3、TLR4、TNF和FOS是宿主对FHV-1免疫反应中潜在的重要基因。这些发现为FHV-1与宿主相互作用的潜在机制提供了有价值的见解。
