猫传染性腹膜炎病毒感染的 CRFK 细胞和猫外周单核细胞中 Toll 样受体 3、7、9 和细胞因子的表达。

Expression of Toll-like receptors 3, 7, 9 and cytokines in feline infectious peritonitis virus-infected CRFK cells and feline peripheral monocytes.

机构信息

Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

出版信息

J Vet Sci. 2022 Mar;23(2):e27. doi: 10.4142/jvs.21225.

DOI:10.4142/jvs.21225

PMID:35363438

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8977543/

Abstract

BACKGROUND

The role of Toll-like receptors (TLRs) in a feline infectious peritonitis virus (FIPV) infection is not completely understood.

OBJECTIVES

This study examined the expression of TLR3, TLR7, TLR9, tumor necrosis factor-alpha (TNF-α), interferon (IFN)-β, and interleukin (IL)-10 upon an FIPV infection in Crandell-Reese feline kidney (CRFK) cells and feline monocytes.

METHODS

CRFK cells and monocytes from feline coronavirus (FCoV)-seronegative cats and FCoV-seropositive cats were infected with type II FIPV-79-1146. At four, 12, and 24 hours post-infection (hpi), the expression of TLR3, TLR7, TLR9, TNF-α, IFN-β, and IL-10, and the viral load were measured using reverse transcription quantitative polymerase chain reaction. Viral protein production was confirmed using immunofluorescence.

RESULTS

FIPV-infected CRFK showed the upregulation of TLR9, TNF-α, and IFN-β expression between 4 and 24 hpi. Uninfected monocytes from FCoV-seropositive cats showed lower TLR3 and TLR9 expression but higher TLR7 expression compared to uninfected monocytes from FCoV-seronegative cats. FIPV-infected monocytes from FCoV-seropositive cats downregulated TLR7 and TNF-α expression between 4 and 24 hpi, and 4 and 12 hpi, respectively. IFN-β was upregulated early in FIPV-infected monocytes from FCoV-seropositive cats, with a significant difference observed at 12 hpi compared to FCoV-seronegative cats. The viral load in the CRFK and FIPV-infected monocytes in both cohorts of cats was similar over time.

CONCLUSION

TLR7 may be the key TLR involved in evading the innate response against inhibiting TNF-α production. Distinct TLR expression profiles between FCoV-seronegative and FCoV-seropositive cats were observed. The associated TLR that plays a role in the induction of IFN-β needs to be explored further.

摘要

背景

Toll 样受体（TLR）在猫传染性腹膜炎病毒（FIPV）感染中的作用尚不完全清楚。

目的

本研究检测了 TLR3、TLR7、TLR9、肿瘤坏死因子-α（TNF-α）、干扰素（IFN）-β和白细胞介素（IL）-10 在猫传染性腹膜炎病毒（FIPV）感染猫肾细胞（CRFK）和猫单核细胞中的表达。

方法

用 FCoV 阴性和 FCoV 阳性猫的 CRFK 细胞和单核细胞感染 FCoV 型 II FIPV-79-1146。在感染后 4、12 和 24 小时（hpi），使用反转录定量聚合酶链反应检测 TLR3、TLR7、TLR9、TNF-α、IFN-β 和 IL-10 的表达和病毒载量。通过免疫荧光法确认病毒蛋白的产生。

结果

FIPV 感染的 CRFK 在 4 至 24 hpi 时 TLR9、TNF-α和 IFN-β 的表达上调。与 FCoV 阴性猫的未感染单核细胞相比，FCoV 阳性猫的未感染单核细胞中 TLR3 和 TLR9 的表达较低，但 TLR7 的表达较高。FCoV 阳性猫的 FIPV 感染单核细胞在 4 至 24 hpi 之间下调 TLR7 和 TNF-α的表达，在 4 至 12 hpi 之间下调 TLR7 的表达。IFN-β 在 FCoV 阳性猫的 FIPV 感染单核细胞中早期上调，在 12 hpi 时与 FCoV 阴性猫相比有显著差异。在两批猫的 CRFK 和 FIPV 感染单核细胞中，病毒载量随时间变化相似。