Meyer Maria Davoli, Coelho Rogério Meneses Ibiapina, Rangel-Coelho João Pedro, Costa Bruna Carolina, Teixeira Lucas Novaes, Martinez Elizabeth Ferreira, Casarin Renato Corrêa Viana, Santamaria Mauro Pedrine, França Fabiana Mantovani Gomes, Nociti-Jr Francisco Humberto, Lisboa-Filho Paulo Noronha, Kantovitz Kamila Rosamilia
Faculdade São Leopoldo Mandic (SLMANDIC), Rua José Rocha Junqueira 13, Swift, Campinas, SP 13045-755, Brazil.
School of Science, State University Júlio de Mesquita (UNESP), Av. Engenheiro Luís Edmundo Carrijo Coube 2085, Bauru, SP 17033-360, Brazil.
Colloids Surf B Biointerfaces. 2025 Feb;246:114389. doi: 10.1016/j.colsurfb.2024.114389. Epub 2024 Nov 20.
The objective was to address the repercussion of adding titanium dioxide nanotubes (TiO-nt) into high-viscosity conventional glass ionomer cement (GIC) on the biological properties of pre-odontoblastic cells (MDPC-23) challenged by lipopolysaccharides (LPS - 2 μg/mL). TiO-nt was added to Ketac Molar EasyMix at 3, 5, 7 %, whereas unblended GIC served as control. Analyses included proliferation (n=6; 24, 48, 72 h), metabolism (MTT; n=6; 24, 48, 72 h); morphology laser microscopy (n=3; 24, 48, 72 h); proteome assessments IL-1β, IL-6, IL-10, VEGF, TNF-α (n=3; 12, 18 h); mRNA levels (RT-PCR) of Il-1β, Il-6, Il-10, VEGF, TNF-α (n=3; 12, 18 h) and DSPP (n=3; 24, 72, 120 h). Data analysis included Shapiro-Wilk, Levene, and generalized linear models (α=0.05). Results demonstrated that cell proliferation increased over time for all groups, and was not impacted by TiO-nt (p>0.05). GIC groups displayed lower MTT values compared to cells cultured without GIC discs (p=0.019); disregarding the presence of TiO. Remarkably, TiO-nt reversed the effect of GIC, reducing the levels of selected biomarkers. LPS treatment modified the expression of the immune-inflammatory markers by MDPC-23 cells (p<0.0001). Morphological analysis did not reveal distinctions for any of the studied. TiO-nt modulated immune-inflammatory and dentin marker expression by MDPC-23 cells cultured on conventional GIC discs, and did not affect cell morphology/viability, regardless LPS exposure. In conclusion, TiO-nt may become a reliable clinical strategy to encourage pulp tissue repair.
目的是探讨在高粘度传统玻璃离子水门汀（GIC）中添加二氧化钛纳米管（TiO-nt）对受脂多糖（LPS - 2μg/mL）刺激的前成牙本质细胞（MDPC-23）生物学特性的影响。将TiO-nt以3%、5%、7%的比例添加到Ketac Molar EasyMix中，未混合的GIC作为对照。分析包括增殖（n=6；24、48、72小时）、代谢（MTT；n=6；24、48、72小时）；形态学激光显微镜检查（n=3；24、48、72小时）；蛋白质组评估IL-1β、IL-6、IL-10、VEGF、TNF-α（n=3；12、18小时）；Il-1β、Il-6、Il-10、VEGF、TNF-α（n=3；12、18小时）和DSPP（n=3；24、72、120小时）的mRNA水平（RT-PCR）。数据分析包括Shapiro-Wilk检验、Levene检验和广义线性模型（α=0.05）。结果表明，所有组细胞增殖均随时间增加，且不受TiO-nt影响（p>0.05）。与未使用GIC盘培养的细胞相比，GIC组MTT值较低（p=0.019）；与TiO的存在无关。值得注意的是，TiO-nt逆转了GIC的作用，降低了所选生物标志物的水平。LPS处理改变了MDPC-23细胞免疫炎症标志物的表达（p<0.0001）。形态学分析未发现任何研究对象之间存在差异。TiO-nt调节了在传统GIC盘上培养的MDPC-23细胞的免疫炎症和牙本质标志物表达，且无论是否暴露于LPS，均不影响细胞形态/活力。总之，TiO-nt可能成为促进牙髓组织修复的可靠临床策略。
