Wang Guoyi, Hu Biao, Yao Xiang, Wei Zhongyan, Chen Jianping, Sun Zongtao
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, Zhejiang, China.
Plant Cell Environ. 2025 Mar;48(3):2329-2342. doi: 10.1111/pce.15308. Epub 2024 Nov 26.
Salivary proteins secreted by phytophagous insects play pivotal roles in plant-insect interactions. A salivary protein RpSP27, from the stinkbug Riptortus pedestris, a devastating pest on soybean, was selected for studying due to its ability to induce cell death and activate immune responses in plants. RpSP27 localized to the endoplasmic reticulum and triggered reactive oxygen species burst. Virus-induced gene silencing assays showed RAR1 plays an essential role in RpSP27-induced cell death in Nicotiana benthamiana. Expression analyses revealed that RpSP27 is predominantly expressed in R. pedestris salivary glands. RNA interference-mediated silencing of RpSP27 in R. pedestris significantly reduced insect survival rates and altered feeding behavior by decreasing the formation of salivary sheaths on soybeans and reducing probing and feeding duration. Furthermore, the silencing of RpSP27 in R. pedestris mitigated the staygreen syndrome in soybeans, characterized by delayed senescence and pod abnormalities. This study elucidated the role of RpSP27 in the interaction between R. pedestris and soybean, presenting a potential target for pest management strategies to protect soybean crops from the detrimental effects of R. pedestris feeding.
植食性昆虫分泌的唾液蛋白在植物与昆虫的相互作用中起着关键作用。从大豆毁灭性害虫豆蝽（Riptortus pedestris）中选取了一种唾液蛋白RpSP27进行研究，因其能够诱导植物细胞死亡并激活植物的免疫反应。RpSP27定位于内质网并引发活性氧爆发。病毒诱导的基因沉默试验表明，RAR1在RpSP27诱导本氏烟草细胞死亡中起重要作用。表达分析显示，RpSP27主要在豆蝽的唾液腺中表达。RNA干扰介导的豆蝽RpSP27沉默显著降低了昆虫的存活率，并通过减少大豆上唾液鞘的形成以及缩短刺探和取食持续时间改变了取食行为。此外，豆蝽中RpSP27的沉默减轻了大豆的持绿综合征，其特征为衰老延迟和豆荚异常。本研究阐明了RpSP27在豆蝽与大豆相互作用中的作用，为害虫管理策略提供了一个潜在靶点，以保护大豆作物免受豆蝽取食的有害影响。
