Hu Biao, Feng Xiuli, Xu Manru, Huang Yue, Guo Chunyun, Yuan Ruikun, Li Yiyuan, Wei Zhongyan, Chen Jianping, Sun Zongtao
State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Rural Affairs (MARA), Zhejiang Key Laboratory of Green Plant Protection, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
Proc Natl Acad Sci U S A. 2025 Feb 4;122(5):e2425190122. doi: 10.1073/pnas.2425190122. Epub 2025 Jan 31.
The stinkbug , notorious for inducing soybean staygreen-like syndrome, employs a range of salivary proteins to manipulate the host plant for its benefit. Here, we show that RpSP1, a salivary protein specific to Pentatomomorpha, triggers plant defense responses in multiple plant species. RpSP1 interacts with and stabilizes a HSP40 family protein GmSPIP1 and is dependent on GmSPIP1 to induce cell death. We show that a critical 22-amino acid peptide within RpSP1 acts as an intracellular insect-derived elicitor. Furthermore, RpSP1 enhances insect-feeding efficiency. The dual functionality of RpSP1 is highlighted by the significant reduction of soybean staygreen-like syndrome following its overexpression in soybean plants or knockdown in insects. Our findings elucidate the complex molecular interactions between plants and herbivores, positioning RpSP1 as a crucial target for developing advanced pest management strategies with broad implications for agricultural biology.
椿象因诱发类似大豆保持绿色综合征而声名狼藉，它利用一系列唾液蛋白来操控宿主植物以谋取自身利益。在此，我们表明，RpSP1是半翅目特有的一种唾液蛋白，可在多种植物物种中触发植物防御反应。RpSP1与热休克蛋白40（HSP40）家族蛋白GmSPIP1相互作用并使其稳定，且依赖GmSPIP1来诱导细胞死亡。我们发现，RpSP1内一段关键的22个氨基酸的肽段可作为一种细胞内昆虫源激发子。此外，RpSP1可提高昆虫的取食效率。在大豆植株中过表达或在昆虫中敲低RpSP1后，大豆保持绿色综合征显著减轻，这凸显了RpSP1的双重功能。我们的研究结果阐明了植物与食草动物之间复杂的分子相互作用，将RpSP1定位为开发先进害虫管理策略的关键靶点，对农业生物学具有广泛影响。
