Jannel Samuel, Caro Yanis, Bermudes Marc, Petit Thomas
Laboratoire de Chimie et de Biotechnologie des Produits Naturels-ChemBioPro (EA2212), Université de La Réunion, 15 Avenue René Cassin, FR-97490 Sainte-Clotilde, La Réunion, France.
Green Mascareignes Technologies SAS, 2 rue Maxime Rivière, FR-97490 Sainte-Clotilde, La Réunion, France.
Foods. 2024 Nov 19;13(22):3681. doi: 10.3390/foods13223681.
is a freshwater green microalgae species able to produce and accumulate astaxanthin in response to environmental stresses such as high light and nutrient deprivation. Astaxanthin is a xanthophyll carotenoid of growing economic interest due to its numerous biological activities, notably its strong antioxidant properties, which can be valued in the fields of nutrition, health, feed and aquaculture. The present study aims at evaluating the capacity of two newly isolated strains from the biodiversity of Reunion Island, to be cultivated in a photobioreactor and to produce astaxanthin. The results showed that both strains were able to grow in various nutritive media and to produce and accumulate astaxanthin in response to stresses, mainly in the form of astaxanthin monoesters, which represented up to 2% of the dry biomass weight and which were mostly composed of linoleic and linolenic acids. In fed-batch cultures using 3 L benchtop photobioreactors, the concentrations of biomass enriched in astaxanthin reached up to 3 g L (dry weight) with biomass productivities of 0.04 and 0.02 g L d based on the durations of the vegetative stage and of the entire culture, respectively. In these cultures, the astaxanthin productivities were found to reach on average around 0.25 mg L d. Although these results were relatively low compared to the literature, the possibility of improving growth conditions in order to improve biomass and astaxanthin yields, to guarantee economic viability for cultivation at a commercial scale, was further discussed.
是一种淡水绿微藻物种，能够在高光和营养缺乏等环境胁迫下产生并积累虾青素。虾青素是一种叶黄素类胡萝卜素，因其众多生物活性，特别是其强大的抗氧化特性，在营养、健康、饲料和水产养殖领域具有越来越高的经济价值。本研究旨在评估从留尼汪岛生物多样性中新分离出的两株菌株在光生物反应器中培养并生产虾青素的能力。结果表明，这两株菌株都能够在各种营养培养基中生长，并在胁迫下产生和积累虾青素，主要以虾青素单酯的形式存在，其含量高达干生物量重量的2%，且主要由亚油酸和亚麻酸组成。在使用3 L台式光生物反应器的分批补料培养中，富含虾青素的生物量浓度分别基于营养生长阶段和整个培养过程的持续时间，达到了3 g/L（干重），生物量生产率分别为0.04 g/L·d和0.02 g/L·d。在这些培养中，发现虾青素生产率平均约为0.25 mg/L·d。尽管与文献相比这些结果相对较低，但进一步讨论了改善生长条件以提高生物量和虾青素产量，从而保证商业规模培养的经济可行性的可能性。
